製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to EDIL3
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Xenopus laevis, Zebrafish, Orangutan
  • 免疫原

    Recombinant fragment: IGYVCKCPRG FNGIHCQHNI NECEVEPCKN GGICTDLVAN YSCECPGEFM GRNCQYKCSG PLGIEGGIIS NQQITASSTH RALFGLQKWY PYYARLNKK, corresponding to amino acids 101-199 of Human EDIL3 protein (NP_005702), with a proprietary tag.

  • ポジティブ・コントロール
    • Partial tagged recombinant Human EDIL3 protein (the immunogen). Human pancreas tissue lysate.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab190692 as a replacement

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 54 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development.
  • 配列類似性
    Contains 3 EGF-like domains.
    Contains 2 F5/8 type C domains.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DEL1 antibody
    • Developmental endothelial locus 1 antibody
    • Developmentally regulated endothelial cell locus 1 protein antibody
    • Developmentally-regulated endothelial cell locus 1 protein antibody
    • Edil3 antibody
    • EDIL3_HUMAN antibody
    • EGF like repeat and discoidin I like domain containing protein 3 antibody
    • EGF like repeats and discoidin I like domains 3 antibody
    • EGF like repeats and discoidin I like domains protein 3 antibody
    • EGF-like repeat and discoidin I-like domain-containing protein 3 antibody
    • Integrin binding protein DEL1 antibody
    • Integrin-binding protein DEL1 antibody
    see all

画像

  • Anti-EDIL3 antibody (ab88667) at 5 µg/ml + Partial tagged recombinant human EDIL3 protein (the immunogen) at 0.2 µg

    Predicted band size: 54 kDa
    Observed band size: 37 kDa (why is the actual band size different from the predicted?)



    Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 37 kDa.
  • Anti-EDIL3 antibody (ab88667) at 5 µg/ml + human pancreas tissue lysate at 50 µg

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

プロトコール

参考文献

ab88667 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Sheep Serum (Seminal plasma and exosomes)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Seminal plasma and exosomes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Tamara Leahy

Verified customer

投稿 Apr 20 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jan 30 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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