Anti-EDG8 抗体 (ab92994)
Key features and details
- Rabbit polyclonal to EDG8
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-EDG8 antibody
EDG8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EDG8 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WBmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse, Dog, Pig -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in the following lysates: Brain: corpus callosum (Human) Whole Cell Lysate; Brain (Human) Tissue Lysate; Spleen (Human) Tissue Lysate; Lung (Human) Tissue Lysate; U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate; MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92994の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 42 kDa).
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 42 kDa). |
ターゲット情報
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機能
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/0)alpha and G(12) subclass of heteromeric G-proteins (By similarity). May play a regulatory role in the transformation of radial glial cells into astrocytes and may affect proliferative activity of these cells. -
組織特異性
Widely expressed in the brain, most prominently in the corpus callosum, which is predominantly white matter. Detected in spleen, peripheral blood leukocytes, placenta, lung, aorta and fetal spleen. Low-level signal detected in many tissue extracts. Overexpressed in leukemic large granular lymphocytes. Isoform 1 is predominantly expressed in peripheral tissues. Isoform 2 is expressed in brain, spleen and peripheral blood leukocytes. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
発生段階
At 24 weeks of gestation, fragments of radial glial fibers are positive within the cortical plate and subplate of allocortical areas. These positive fragments often appear enlarged as varicosities and some of them terminate at blood vessels. Between 28 and 30 weeks of gestation, all iso- and allocortical areas contain immunolabelled radial glial fibers revealing curvature next to sulci. After 32 weeks of gestation, radial glial fibers gradually disappear; instead positive transitional stages between radial glia and astrocytes were found. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 53637 Human
- Entrez Gene: 94226 Mouse
- Entrez Gene: 60399 Rat
- Omim: 605146 Human
- SwissProt: Q9H228 Human
- SwissProt: Q91X56 Mouse
- SwissProt: Q9JKM5 Rat
- Unigene: 501561 Human
see all -
別名
- EDG 8 antibody
- EDG8 S1P receptor antibody
- Endothelial differentiation G protein coupled receptor 8 antibody
see all
画像
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All lanes : Anti-EDG8 antibody (ab92994) at 1 µg/ml
Lane 1 : Human brain corpus callosum tissue lysate (alzheimer) - total protein (ab30027)
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lane 3 : Human spleen tissue lysate - total protein (ab29699)
Lane 4 : Lung (Human) Tissue Lysate
Lane 5 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
Lane 6 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab92994 は 3 報の論文で使用されています。
- Gläser A et al. Identification of Brain-Specific Treatment Effects in NPC1 Disease by Focusing on Cellular and Molecular Changes of Sphingosine-1-Phosphate Metabolism. Int J Mol Sci 21:N/A (2020). PubMed: 32599915
- Palangi A et al. Differential expression of S1P receptor subtypes in human bladder transitional cell carcinoma. Clin Transl Oncol N/A:N/A (2019). PubMed: 30712233
- Arana L et al. Ceramide 1-phosphate induces macrophage chemoattractant protein-1 release: involvement in ceramide 1-phosphate-stimulated cell migration. Am J Physiol Endocrinol Metab 304:E1213-26 (2013). PubMed: 23548612