製品の概要

  • 製品名Anti-EDG2 antibody
    EDG2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EDG2
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human EDG2.

    (Peptide available as ab94879.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Human brain; Human pancreas; Human kidney; HeLa whole cell; Jurkat whole cell; A549 whole cell

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab84788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa).

ターゲット情報

  • 機能Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation.
  • 組織特異性Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在Cell surface. Cell membrane. Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5031439C20 antibody
    • AI326300 antibody
    • EDG 2 antibody
    • Endothelial differentiation gene 2 antibody
    • GPCR 26 antibody
    • Gpcr26 antibody
    • Gpcr91 antibody
    • Kdt2 antibody
    • LPA 1 antibody
    • LPA receptor 1 antibody
    • LPA receptor EDG2 antibody
    • LPA-1 antibody
    • lpA1 antibody
    • Lpar1 antibody
    • LPAR1_HUMAN antibody
    • Lysophosphatidic acid receptor 1 antibody
    • Lysophosphatidic acid receptor Edg-2 antibody
    • Lysophosphatidic acid receptor EDG2 antibody
    • MGC105279 antibody
    • MGC29102 antibody
    • Mrec1.3 antibody
    • rec.1.3 antibody
    • Ventricular zone gene 1 antibody
    • vzg-1 antibody
    • VZG1 antibody
    see all

Anti-EDG2 antibody 画像

  • All lanes : Anti-EDG2 antibody (ab84788) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ICC/IF image of ab84788 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84788, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-EDG2 antibody (ab84788) 使用論文

ab84788 has not yet been referenced specifically in any publications.

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