Anti-S1P1/EDG1 抗体 (ab11424)
Key features and details
- Rabbit polyclonal to S1P1/EDG1
- Suitable for: IP, ICC/IF, IHC-P, WB, Flow Cyt, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-S1P1/EDG1 antibody
S1P1/EDG1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to S1P1/EDG1 -
由来種
Rabbit -
特異性
ab11424 detects S1P1/EDG1 protein in transfected human cell samples. This antibody shows no cross-reactivity to S1P3. -
アプリケーション
適用あり: IP, ICC/IF, IHC-P, WB, Flow Cyt, ICCmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Horse, Cow, Cat, Monkey -
免疫原
Synthetic peptide corresponding to Mouse S1P1/EDG1 aa 359-372.
Sequence:SHPQKDDGDNPETI
(Peptide available asab39763) -
ポジティブ・コントロール
- WB: Rat brain tissue lysate; Neuro-2a cell lysate. ICC: CCL39 and Neuro-2a cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11424の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa. Band represents recombinant S1P1 from transfected HEK293 cells.
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Flow Cyt |
Use at an assay dependent concentration. PubMed: 20733200
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC |
Use a concentration of 2 µg/ml.
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特記事項 |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa. Band represents recombinant S1P1 from transfected HEK293 cells. |
Flow Cyt
Use at an assay dependent concentration. PubMed: 20733200 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC
Use a concentration of 2 µg/ml. |
ターゲット情報
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機能
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins. -
組織特異性
Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
翻訳後修飾
S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 281135 Cow
- Entrez Gene: 100050049 Horse
- Entrez Gene: 1901 Human
- Entrez Gene: 13609 Mouse
- Entrez Gene: 29733 Rat
- Omim: 601974 Human
- SwissProt: Q5E9P3 Cow
- SwissProt: P21453 Human
see all -
別名
- CD363 antibody
- CHEDG 1 antibody
- CHEDG1 antibody
see all
画像
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Lane 1 : Anti-S1P1/EDG1 antibody (ab11424) at 2 µg/ml
Lane 2 : Anti-S1P1/EDG1 antibody (ab11424) at 2 mg/ml
Lane 1 : Rat brain tissue extract
Lane 2 : Neuro-2a Cells
Secondary
All lanes : Goat anti-rabbit IgG (H+L), HRP conjugated at 0.4 µg/ml
Developed using the ECL technique. -
Immunocytochemical analysis of Neuro-2a cells, labeling S1P1/EDG1 with ab11424. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. Immunostaining with anti-EDG1 diluted 2µg/ml in 0.1% BSA for 3 hours at room temperature.
Green staining - ab11424
Blue staining - DAPI for nuclear localization
Red staining - F-actin
The right-most image is a control (no primary antibody staining).
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ab11424 staining S1P1/EDG1 in transfected CCL39 cells by Immunocytochemistry/ Immunofluorescence.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (28)
ab11424 は 28 報の論文で使用されています。
- Xu (徐兴丽) X et al. Knockout of the NONO Gene Inhibits Neointima Formation in a Mouse Model of Vascular Injury. Arterioscler Thromb Vasc Biol 41:1428-1445 (2021). PubMed: 33626912
- Hampson E et al. P-Rex1 Controls Sphingosine 1-Phosphate Receptor Signalling, Morphology, and Cell-Cycle Progression in Neuronal Cells. Cells 10:N/A (2021). PubMed: 34572121
- Yang XX et al. Molecular Mechanism of Sphingosine-1-Phosphate Receptor 1 Regulating CD4+ Tissue Memory in situ T Cells in Primary Sjogren's Syndrome. Int J Gen Med 14:6177-6188 (2021). PubMed: 34611431
- Matsumoto N et al. Up-regulation of sphingosine-1-phosphate receptors and sphingosine kinase 1 in the peri-ischemic area after transient middle cerebral artery occlusion in mice. Brain Res 1739:146831 (2020). PubMed: 32278723
- Zou Y et al. Non-Mitogenic Fibroblast Growth Factor 1 Enhanced Angiogenesis Following Ischemic Stroke by Regulating the Sphingosine-1-Phosphate 1 Pathway. Front Pharmacol 11:59 (2020). PubMed: 32194396