製品の概要

  • 製品名Anti-EDF1 antibody
    EDF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EDF1
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (AESDWDTVTV LRKKGPTAAQ AKSKQAILAA QRRGEDVETS KKWAAGQNKQ) of Human EDF1 (NP_003783).

  • ポジティブ・コントロール
    • THP1 cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab95110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.
  • 組織特異性Expressed in brain, liver, lung, kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart, pancreas, liver, intestine and adipose tissues.
  • 配列類似性Contains 1 HTH cro/C1-type DNA-binding domain.
  • 発生段階Expressed in fetal tissues. More abundant in kidney.
  • ドメインThe IQ motif, which is involved in calmodulin binding, overlaps with the binding domain for nuclear receptors and transcription factors. Its phosphorylation probably allows a switch between the two activities of the protein.
  • 翻訳後修飾Phosphorylated (by PKA and PKC).
  • 細胞内局在Cytoplasm. Nucleus. Also nuclear upon binding to NR5A1 and treatment of cells with TPA or forskolin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAP19 antibody
    • EDF-1 antibody
    • edf1 antibody
    • EDF1_HUMAN antibody
    • Endothelial differentiation related factor 1 antibody
    • Endothelial differentiation-related factor 1 antibody
    • MBF1 antibody
    • MGC9058 antibody
    • Multiprotein bridging factor 1 antibody
    • Multiprotein-bridging factor 1 antibody
    see all

Anti-EDF1 antibody 画像

  • Immunofluorescence analysis of human fibroblasts fixed with methanol labelling EDF1 with ab95110 at 5ug/ml.

  • Anti-EDF1 antibody (ab95110) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 16 kDa
    Gel concentration: 10-20%

Anti-EDF1 antibody (ab95110) 使用論文

ab95110 has not yet been referenced specifically in any publications.

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