Anti-EDD 抗体 (ab70311)
Key features and details
- Rabbit polyclonal to EDD
- Suitable for: IHC-P, WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-EDD antibody
EDD 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EDD -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IP, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus -
免疫原
Synthetic peptide corresponding to a region between residues 600 and 650 of human EDD. (NP_056986.2 )
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ポジティブ・コントロール
- HeLa and NIH3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab70311 was affinity purified using an epitope specific to EDD immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70311の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 309 kDa (predicted molecular weight: 309 kDa).
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IP |
Use at 2-10 µg/mg of lysate.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Detects a band of approximately 309 kDa (predicted molecular weight: 309 kDa). |
IP
Use at 2-10 µg/mg of lysate. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. -
組織特異性
Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 PABC domain.
Contains 1 UBR-type zinc finger. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 515582 Cow
- Entrez Gene: 51366 Human
- Entrez Gene: 70790 Mouse
- Entrez Gene: 100157625 Pig
- Entrez Gene: 117060 Rat
- Omim: 608413 Human
- SwissProt: O95071 Human
- SwissProt: Q80TP3 Mouse
see all -
別名
- 4432411E13Rik antibody
- AW549941 antibody
- C77315 antibody
see all
画像
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All lanes : Anti-EDD antibody (ab70311) at 0.4 µg/ml
Lane 1 : HEK293T whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : LNCaP whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 309 kDa
Exposure time: 3 minutes -
Immunoprecipitation of HeLa cells using ab70311
Lane 1: ab70311 current lot
Lane 2: ab70311 previous lot
Lane 3: Control IgG
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Immunohistochemistry analysis of formalin-fixed paraffin-embedded sections of mouse plasmacytoma staining EDD with ab70311 at 1/1000 dilution
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Immunohistochemistry analysis of formalin-fixed paraffin-embedded sections of human lung carcinoma staining EDD with ab70311 at 1/1000 dilution
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab70311 は 10 報の論文で使用されています。
- Wang M et al. Targeting UBR5 in hepatocellular carcinoma cells and precise treatment via echinacoside nanodelivery. Cell Mol Biol Lett 27:92 (2022). PubMed: 36224534
- Wang K et al. UBR5 regulates proliferation and radiosensitivity in human laryngeal carcinoma via the p38/MAPK signaling pathway. Oncol Rep 44:685-697 (2020). PubMed: 32468011
- Duan Z et al. TMT-based quantitative proteomics analysis reveals the attenuated replication mechanism of Newcastle disease virus caused by nuclear localization signal mutation in viral matrix protein. Virulence 11:607-635 (2020). PubMed: 32420802
- Khan MM et al. Protein interactome of the Cancerous Inhibitor of protein phosphatase 2A (CIP2A) in Th17 cells. Curr Res Immunol 1:10-22 (2020). PubMed: 33817627
- Ding F et al. UBR5 oncogene as an indicator of poor prognosis in gastric cancer. Exp Ther Med 20:7 (2020). PubMed: 32934672
- Xie Z et al. Significance of the E3 ubiquitin protein UBR5 as an oncogene and a prognostic biomarker in colorectal cancer. Oncotarget 8:108079-108092 (2017). PubMed: 29296225
- Flack JE et al. Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Mol Cell 67:181-193.e5 (2017). PubMed: 28689657
- Liao L et al. E3 Ubiquitin Ligase UBR5 Drives the Growth and Metastasis of Triple-Negative Breast Cancer. Cancer Res 77:2090-2101 (2017). PubMed: 28330927
- Bolt MJ et al. Systems level-based RNAi screening by high content analysis identifies UBR5 as a regulator of estrogen receptor-a protein levels and activity. Oncogene N/A:N/A (2014). Human . PubMed: 24441042
- Morrison J et al. Dengue virus co-opts UBR4 to degrade STAT2 and antagonize type I interferon signaling. PLoS Pathog 9:e1003265 (2013). WB ; Human . PubMed: 23555265