製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to EDD
  • アプリケーション
    適用あり: WB, IP, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Human
    交差が予測される動物種: Cow, Pig
  • 免疫原

    Synthetic peptide: LAIKTKNFGFV, corresponding to C terminal amino acids 2789-2799 of Human EDD.

  • 特記事項
    GenBank Accession Number – NP_056986. LocusLink ID - 51366 (human); 70790 (mouse); 117060 (rat). Gene Ontology terms - ubiquitin conjugating enzyme activity; ubiquitin-protein ligase activity; ubiquitin cycle; cell proliferation; protein modification; soluble fraction; nucleus; ligase activity.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab4376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 309 kDa (predicted molecular weight: 309 kDa).Can be blocked with Human EDD peptide (ab23029).
IP Use at an assay dependent dilution.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. No results could not be obtained after antigen retrieval at pH6.
ICC/IF Use at an assay dependent concentration. PubMed: 21148289

ターゲット情報

  • 機能
    E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development.
  • 組織特異性
    Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 PABC domain.
    Contains 1 UBR-type zinc finger.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4432411E13Rik antibody
    • AW549941 antibody
    • C77315 antibody
    • D030042K14 antibody
    • DD5 antibody
    • E3 identified by differential display antibody
    • E3 ubiquitin protein ligase, HECT domain containing, 1 antibody
    • E3 ubiquitin-protein ligase antibody
    • E3 ubiquitin-protein ligase UBR5 antibody
    • EDD 1 antibody
    • EDD antibody
    • EDD1 antibody
    • FLJ11310 antibody
    • HECT domain-containing 1 antibody
    • hHYD antibody
    • HYD antibody
    • Hyperplastic discs protein homolog antibody
    • Hyperplastic discs, Drosophila, homolog of antibody
    • KIAA0896 antibody
    • MGC57263 antibody
    • mKIAA0896 antibody
    • Progestin induced protein antibody
    • Progestin-induced protein antibody
    • Rat100 antibody
    • Ubiquitin protein ligase antibody
    • Ubiquitin protein ligase E3 component n recognin 5 antibody
    • UBR5 antibody
    • UBR5_HUMAN antibody
    see all

画像

  • Anti-EDD antibody (ab4376) at 1/500 dilution + MDA-MB-468 breast cancer cells (25µg)

    Secondary
    Donkey anti-goat conjugated to HRP at 1/4000 dilution

    Performed under reducing conditions.

    Predicted band size : 309 kDa


    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab4376 (4µg/ml) staining of paraffin embedded Human Testis shows nuclear staining . Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.

参考文献

This product has been referenced in:
  • Hay-Koren A  et al. The EDD E3 ubiquitin ligase ubiquitinates and up-regulates beta-catenin. Mol Biol Cell 22:399-411 (2011). WB ; Human . Read more (PubMed: 21118991) »
  • Won JH  et al. Phenotypic changes in mouse pancreatic stellate cell Ca2+ signaling events following activation in culture and in a disease model of pancreatitis. Mol Biol Cell 22:421-36 (2011). ICC/IF . Read more (PubMed: 21148289) »

See all 2 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
50 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (7% Tris-Bicine Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Verified customer

投稿 Sep 28 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Rat Tissue lysate - whole (lung tissue)
Total protein in input
1000 µg
Specification
lung tissue
Immuno-precipitation step
Protein A/G
Username

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Verified customer

投稿 Oct 31 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Rabbit Tissue lysate - whole (heart tissue)
Total protein in input
1000 µg
Specification
heart tissue
Immuno-precipitation step
Protein A/G
Username

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Verified customer

投稿 Oct 31 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (lung tissue)
Total protein in input
1000 µg
Specification
lung tissue
Immuno-precipitation step
Protein A/G
Username

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Verified customer

投稿 Oct 20 2008

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Tissue lysate - whole (heart)
Loading amount
50 µg
Specification
heart
Gel Running Conditions
Reduced Denaturing (6% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Aug 12 2008

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3)
Loading amount
30 µg
Specification
NIH 3T3
Gel Running Conditions
Reduced Denaturing (6% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jun 12 2008

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (C6 cell line)
Loading amount
25 µg
Specification
C6 cell line
Gel Running Conditions
Reduced Denaturing (4% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jun 04 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (breast cancer cells)
Total protein in input
250 µg
Specification
breast cancer cells
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

投稿 May 23 2008

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (T98G cell line)
Loading amount
25 µg
Specification
T98G cell line
Gel Running Conditions
Reduced Denaturing (6% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

投稿 May 02 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-468 breast cancer cells)
Loading amount
25 µg
Specification
MDA-MB-468 breast cancer cells
Gel Running Conditions
Reduced Denaturing
Blocking step
SuperBlock Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

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Verified customer

投稿 Mar 12 2008

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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