Anti-EDC4 抗体 (ab72408)
Key features and details
- Rabbit polyclonal to EDC4
- Suitable for: ICC/IF, WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-EDC4 antibody
EDC4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EDC4 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human EDC4.
Database link: Q6P2E9 -
ポジティブ・コントロール
- WB: HeLa, HEK293T, Jurkat, NIH 3T3 and TCMK-1 cells. IHC-P: human normal skin. IP: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72408の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (2) |
Use at an assay dependent concentration. PubMed: 19729507
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WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 55, 190 kDa (predicted molecular weight: 152 kDa).
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IP | (2) |
Use at 2-5 µg/mg of lysate.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. PubMed: 19729507 |
WB
1/2000 - 1/10000. Detects a band of approximately 55, 190 kDa (predicted molecular weight: 152 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro). -
配列類似性
Belongs to the WD repeat EDC4 family.
Contains 4 WD repeats. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm > P-body. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 23644 Human
- Entrez Gene: 234699 Mouse
- Entrez Gene: 701393 Rhesus monkey
- Omim: 606030 Human
- SwissProt: Q6P2E9 Human
- SwissProt: Q3UJB9 Mouse
- Unigene: 75682 Human
- Unigene: 28979 Mouse
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別名
- autoantigen antibody
- Autoantigen Ge 1 antibody
- Autoantigen Ge-1 antibody
see all
画像
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Immunoprecipitation/ Western Blot of EDC4. Lane 1: ab72408 at 6µg/mg whole cell lysate. Lane 2: Different lot of ab72408 at 6µg/ml for WB. Lane 3: Control IgG. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 3 minutes.
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All lanes : Anti-EDC4 antibody (ab72408) at 1/2000 dilution
Lane 1 : NIH 3T3 Whole cell lysate
Lane 2 : TCMK-1 Whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 152 kDa -
All lanes : Anti-EDC4 antibody (ab72408) at 1/2000 dilution
Lane 1 : HeLa Whole cell lysate
Lane 2 : HEK293T Whole cell lysate
Lane 3 : Jurkat Whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 152 kDa -
IHC image of ab72408 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72408, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab72408 は 13 報の論文で使用されています。
- Hallacli E et al. The Parkinson's disease protein alpha-synuclein is a modulator of processing bodies and mRNA stability. Cell 185:2035-2056.e33 (2022). PubMed: 35688132
- Fan S et al. Enterovirus 71 2A Protease Inhibits P-Body Formation To Promote Viral RNA Synthesis. J Virol 95:e0092221 (2021). PubMed: 34287048
- Di Stefano B et al. The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis. Cell Stem Cell 25:622-638.e13 (2019). PubMed: 31588046
- Hernández G et al. Decapping protein EDC4 regulates DNA repair and phenocopies BRCA1. Nat Commun 9:967 (2018). PubMed: 29511213
- Namkoong S et al. Systematic Characterization of Stress-Induced RNA Granulation. Mol Cell 70:175-187.e8 (2018). PubMed: 29576526