製品の概要

  • 製品名Anti-EDC3 antibody
    EDC3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to EDC3
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-509 of Human EDC3

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab57780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Binds single-stranded RNA. In the process of mRNA degradation, may play a role in mRNA decapping. May play a role in spermiogenesis and oogenesis.
  • 組織特異性Expressed in theca and granulosa cells in ovary, and in spermatids of the meiotic division part II and apical membrane of Sertoli cells in testis (at protein level). Also expressed in brain and mammary gland.
  • 配列類似性Belongs to the EDC3 family.
    Contains 1 DFDF domain.
    Contains 1 YjeF N-terminal domain.
  • ドメインThe DFDF domain is unstructured by itself. It assumes a helical fold upon interaction with DDX6.
  • 細胞内局在Cytoplasm > P-body. Processing bodies.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EDC 3 antibody
    • edc3 antibody
    • EDC3_HUMAN antibody
    • enhancer of mRNA decapping 3 homolog (S. cerevisiae) antibody
    • Enhancer of mRNA decapping 3 homolog antibody
    • Enhancer of mRNA decapping 3, S. cerevisiae, homolog of antibody
    • Enhancer of mRNA decapping protein 3 antibody
    • Enhancer of mRNA-decapping protein 3 antibody
    • FLJ21128 antibody
    • FLJ31777 antibody
    • hYjeF_N2 antibody
    • hYjeF_N2-15q23 antibody
    • LSM 16 antibody
    • LSM16 antibody
    • LSM16 homolog (EDC3 S. cerevisiae) antibody
    • LSM16 homolog (EDC3, S. cerevisiae) antibody
    • LSM16 homolog antibody
    • YJDC antibody
    • YjeF domain containing antibody
    • YjeF domain containing protein 1 antibody
    • YjeF domain containing protein antibody
    • YjeF domain-containing protein 1 antibody
    • YjeF N-terminal domain-containing protein 2 antibody
    • YjeF_N2 antibody
    • YJEFN2 antibody
    see all

Anti-EDC3 antibody 画像

  • Overlay histogram showing HeLa cells stained with ab57780 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57780, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • EDC3 antibody (ab57780) at 1ug/lane + HeLa cell lysate at 25ug/lane.

Anti-EDC3 antibody (ab57780) 使用論文

This product has been referenced in:

See all 4 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab57780.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"