製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ECSIT
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Human
  • 免疫原

    Synthetic peptide: TGMEDPTQPH IVGIQSPDQQ AALARHNPSR PVFVEGPFPL WLRNKCVYYH , corresponding to amino acids 254-303 of Human ECSIT

  • ポジティブ・コントロール
    • NIH 3T3 lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using a peptide based assay was 1/1562500.

ターゲット情報

  • 機能
    Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity). Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase.
  • 配列類似性
    Belongs to the ECSIT family.
  • 細胞内局在
    Cytoplasm. Nucleus. Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ecsit antibody
    • ECSIT homolog antibody
    • ECSIT protein antibody
    • ECSIT_HUMAN antibody
    • Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial antibody
    • Evolutionarily conserved signaling intermediate in Toll pathways antibody
    • Likely ortholog of mouse signaling intermediate in Toll pathway evolutionarily conserved antibody
    • Protein SITPEC antibody
    • Signaling intermediate in Toll pathway evolutionarily conserved antibody
    • SITPEC antibody
    • SITPEC protein antibody
    see all

画像

  • Anti-ECSIT antibody (ab66380) at 1.25 µg/ml + NIH 3T3 lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab66380 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab66380.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録