製品の概要

  • 製品名Anti-ECM29 antibody
    ECM29 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ECM29
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human ECM29 aa 1821-1839 (C terminal).
    Sequence:

    RPELQEKAALLKKTLENLE

  • ポジティブ・コントロール
    • WB: HeLa cell lysate. ICC/IF: HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: Whole serum
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab28666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
WB 1/200. Detects a band of approximately 205 kDa (predicted molecular weight: 205 kDa).

ターゲット情報

  • 関連性ECM29 appears to be one of three proteins which associate significantly with proteasomes. ECM29 has been shown to enhance the stability of the proteasome. ECM29 deletion has been shown to produce 26S proteasomes which dissociated in the absence of ATP. It was thus suggested that ECM29 tethers the regulatory complex to the proteasome.
  • 細胞内局在Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Endosome. Cytoplasm › cytoskeleton › centrosome. Nucleus.
  • 参照データベース
  • 別名
    • ECM29 homolog, proteasome accessory protein antibody
    • FLJ22036 antibody
    • KIAA0368 antibody
    • KIAA1962 antibody
    • Proteasome associated protein ECM29 homolog antibody
    • RP11-386D8.2 antibody
    see all

Anti-ECM29 antibody 画像

  • ab28666 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28666 at 1µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-ECM29 antibody (ab28666) (dilution of 1/200) + HeLa cell extract

    Predicted band size : 205 kDa
    Observed band size : 205 kDa
    Detection of ECM29 in HeLa cells.
  • Immunofluorescent staining of Ecm29 in HeLa cells using ab28666 (1/200 dilution).

Anti-ECM29 antibody (ab28666) 使用論文

ab28666 has not yet been referenced specifically in any publications.

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