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The ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. The ECL Western Blotting Substrate offers superior signal and clear background. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed.
Looking for our new high sensitivity Optiblot ECL kits. View them here.
Reagents provided are sufficient for 400 cm2 of membrane.
|Reagent A||1 x 25ml|
|Reagent B||1 x 25ml|
Our Abpromise guarantee covers the use of ab65623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|