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human liver mitochondria
ECH1 is a member of the hydratase/isomerase superfamily involved in fatty acid metabolism. It is localized to peroxisomes and/or mitochondria in different mammals. In humans, levels of ECH1 are modulated by peroxisome proliferators. As the name implies, ECH1 catalyzes the isomerization of cis ?3,5, double bonds in unsaturated acyl-CoAs to yield trans ?2,4-dienoyl CoAs. These are substrates for ?2,4-dienoyl CoA reductase which produces trans ?3 enoyl CoA. This intermediate can then be further transformed by ?3, ?2 - enoyl CoA isomerase to finally yield the trans-2 double bond configured fatty acyl CoA, which can be then directed into the ß-oxidation pathway. Expression of ECH1 is highest in metabolically active tissues such as heart, skeletal muscle, central nervous system, liver and kidney. Recent studies show that it is down regulated in gastric carcinoma and CLL.
Product was previously marketed under the MitoSciences sub-brand.
Our Abpromise guarantee covers the use of ab110294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 4 µg/ml.|
|In-Cell ELISA||Use a concentration of 4 µg/ml.|
|Flow Cyt||Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
ab110294 has not yet been referenced specifically in any publications.