Anti-ECH1 抗体 [3B7CF2AC8] (ab123450)
Key features and details
- Mouse monoclonal [3B7CF2AC8] to ECH1
- Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
- Reacts with: Rat, Cow, Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-ECH1 antibody [3B7CF2AC8]
ECH1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3B7CF2AC8] to ECH1 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, IHC-P, WB, Flow Cytmore details -
種交差性
交差種: Rat, Cow, Human -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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ポジティブ・コントロール
- HDFn cells; Human, Rat and Cow heart homogenates; Human liver homogenate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading...
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精製度
IgG fraction -
特記事項(精製)
Purity is near homogeneity as judged by SDS-PAGE. ab123450 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
ポリ/モノ
モノクローナル -
クローン名
3B7CF2AC8 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab123450の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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IHC-P |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA. -
パスウェイ
Lipid metabolism; fatty acid beta-oxidation. -
配列類似性
Belongs to the enoyl-CoA hydratase/isomerase family. -
細胞内局在
Mitochondrion. Peroxisome. - Information by UniProt
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参照データベース
- Entrez Gene: 507431 Cow
- Entrez Gene: 1891 Human
- Entrez Gene: 64526 Rat
- Omim: 600696 Human
- SwissProt: Q13011 Human
- SwissProt: Q62651 Rat
- Unigene: 196176 Human
- Unigene: 6148 Rat
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別名
- 4)-dienoyl-CoA isomerase antibody
- 5)-Delta(2 antibody
- Delta(3 5) Delta(2 4) dienoyl CoA isomerase antibody
see all
画像
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All lanes : Anti-ECH1 antibody [3B7CF2AC8] (ab123450) at 1 µg/ml
Lane 1 : Human heart homogenate
Lane 2 : Human liver homogenate
Lane 3 : Rat heart homogenate
Lane 4 : Cow heart homogenate
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : Goat-anti-Mouse HRP
Predicted band size: 36 kDa -
ab123450 at 1µg/ml staining ECH1 in 4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized HDFn cells by Immunocytochemistry followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). DAPI was used to stain the cell nuclei (blue).
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ab123450 at 1µg/ml staining ECH1 in HDFn cells by Flow Cytometry (blue) or no primary antibody control (red).
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IHC image of ECH1 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab123450, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab123450 は論文での使用が確認できていません。