製品の概要

  • 製品名Anti-EBV LMP2A antibody [14B7]
    EBV LMP2A 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [14B7] to EBV LMP2A
  • 特異性ab59026 is specific for Epstein-Barr virus LMP2A. Clone 14B7 detects wild-type LMP2A protein.
  • アプリケーション適用あり: WB, IP, ICC/IFmore details
    適用なし: IHC-P
  • 種交差性
    Epstein-Barr virus
  • 免疫原

    Bacterial TrpE-LMP2A fusion protein

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名14B7
  • ミエローマP3x63-Ag8.653
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/1000. Detects a band of approximately 50 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 関連性Epstein Barr virus (EBV) is a member of the herpesvirus family and one of the most common human viruses. Most people become infected with EBV during their lives. Primary infections usually results in infectious mononucleosis (glandular fever) but the virus can also lay dormant in B lymphocytes and when reactivated become associated with more serious disease such as Burkitt's lymphoma, nasopharyngeal carcinoma and Hodgkin's disease. EBV latently infects B lymphocytes. Infected B cells express EBV nuclear antigens and latent proteins LMP1, LMP2A and LMP2B. LMP2A forms aggregates in the plasma membranes of B lymphocytes, where it functions as a negative regulator of the Src and Syk protein tyrosine kinases. Studies show that LMP2A blocks B-cell receptor (BCR) signal transduction in EBV immortalized B cells in vitro and may play an important role in maintaining a latent EBV infection within the peripheral blood B cells of infected individuals.
    • 別名
      • EBV latent membrane protein 2A antibody
      • Epstein Barr virus antibody
      • HHV4 antibody
      • Human Herpesvirus 4 antibody
      • Latent membrane protein 2 antibody
      • LMP2 antibody
      • Terminal protein antibody
      see all

    Anti-EBV LMP2A antibody [14B7] (ab59026) 使用論文

    This product has been referenced in:
    • Minamitani T  et al. Evasion of affinity-based selection in germinal centers by Epstein-Barr virus LMP2A. Proc Natl Acad Sci U S A 112:11612-7 (2015). WB ; Mouse . Read more (PubMed: 26305967) »
    • Marquitz AR  et al. Expression profile of microRNAs in Epstein-Barr virus-infected AGS gastric carcinoma cells. J Virol 88:1389-93 (2014). WB . Read more (PubMed: 24227849) »

    See all 6 Publications for this product

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    Thank you for your enquiry.

    I am sorry to confirm that because we carry over 90,000 products, it is regrettably not feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (B cell)
    Loading amount 10 µg
    Specification B cell
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    Verified customer

    投稿 Jun 21 2010

    Thank you for your enquiry. The size of protein detected can vary from 45 kDa to around 66 kDa depending on the level of phosphorylation. Unfortunately we do not have any WB images available but the two papers below using the same clone, will provi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"