Anti-EBV Latent Membrane Protein 1 抗体 [CS 1-4] (ab78113)

製品の概要

  • 製品名
    Anti-EBV Latent Membrane Protein 1 antibody [CS 1-4]
    EBV Latent Membrane Protein 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CS 1-4] to EBV Latent Membrane Protein 1
  • 特異性
    This antibody is specific for Epstein-Barr virus infected cells expressing the latent membrane protein (LMP). This is in fact four pooled antibodies which collectively detect LMP's encoded by each of 20 geographically distinct EBV isolates.
  • アプリケーション
    適用あり: WB, IP, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Epstein-Barr virus
  • 免疫原

    Fusion protein

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    モノクローナル
  • クローン名
    CS 1-4
  • ミエローマ
    x63-Ag8.653
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab78113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    EBV is a human herpesvirus that establishes a life-long persistence in the host. The virus infects the vast majority of the world's adult population and is well known for its association with a broad spectrum of benign and malignant diseases, including infectious mononucleosis, Burkitt's lymphoma, nasopharyngeal carcinoma, and is causally associated with lymphoid and epithelial malignancies, including post-transplant lymphoproliferative disorders, Hodgkin's disease, anaplastic nasopharyngeal carcinoma and gastric carcinomas. Latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is a transforming protein that affects multiple cell signalling pathways and contributes to EBV-associated oncogenesis. LMP1 can be expressed in some states of EBV latency, and significant induction of full-length LMP1 is also observed frequently during virus reactivation into the lytic cycle. LMP1 is critical for EBV-infected cell activation, adhesion and survival, and is usually expressed in the malignant cells.
  • 参照データベース
    • 別名
      • BNLF1 antibody
      • EBV antibody
      • Epstein Barr virus antibody
      • HHV4 antibody
      • Human Herpesvirus 4 antibody
      • Latent membrane protein 1 antibody
      • LMP1 antibody
      see all

    参考文献

    This product has been referenced in:
    • Martin KA  et al. Epstein-Barr Virus Oncoprotein LMP1 Mediates Epigenetic Changes in Host Gene Expression through PARP1. J Virol 90:8520-30 (2016). Read more (PubMed: 27440880) »
    • Campion EM  et al. Repression of the proapoptotic cellular BIK/NBK gene by Epstein-Barr virus antagonizes transforming growth factor ß1-induced B-cell apoptosis. J Virol 88:5001-13 (2014). Read more (PubMed: 24554662) »

    See all 9 Publications for this product

    レビューと Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (EBV transformed B cells)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    10 µg
    Specification
    EBV transformed B cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    投稿 Jul 08 2015

    Application
    Immunocytochemistry
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 20°C
    Sample
    Mouse Cultured Cells (lymphocyte)
    Specification
    lymphocyte
    Permeabilization
    Yes - acetone
    Fixative
    Methanol
    Username

    Abcam user community

    Verified customer

    投稿 Jan 23 2015

    Application
    Western blot
    Loading amount
    100 µg
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-12% gel)
    Sample
    Human Cell lysate - whole cell (PBMC)
    Specification
    PBMC
    Treatment
    EBV for 14days
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jan 23 2015

    Application
    Immunocytochemistry
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Sample
    Mouse Cultured Cells (lymphoma)
    Specification
    lymphoma
    Permeabilization
    Yes - Acetone
    Fixative
    Methanol
    Username

    Abcam user community

    Verified customer

    投稿 Jan 23 2015

    Vielen Dank für Ihre Antwort.

    Leider muss ich Ihnen bestätigen, dass wir keinen Antikörper gegen die extrazelluläre Domäne in Produktion haben.

    Ich werde diesen Vorschlag allerdings gerne weitergeben. Leider...

    Read More

    Thank you for contacting Abcam. I have tried blasting the human EBV-LMP1 sequences against the rhesus monkey database and I cannot seem to get a hit for the rhesus EBV sequence (the only hit is for unnamed protein product, #21103, which has a 70% homol...

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    Thank you for contacting us. The secondary antibody ab6785 (goat anti-mouse IgG, FITC-conjugated) is a suitable secondary for the three primary antibodies you asked about (ab78113, ab90543, and ab3280), as well as ab6276. I hope this inf...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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