製品の概要

  • 製品名
    Anti-Ebola virus antibody [FE37]
    Ebola virus 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [FE37] to Ebola virus
  • 特異性
    Ebola virus, Zaire strain In Western blot they stain a single viral protein band corresponding to the envelope protein GP with a molecular mass of 74.5 kDa.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 免疫原

    Formalin inactivated Ebola virus (strain Zaire).

  • 特記事項
    Concentration varies from lot to lot and can be provided on request.


    Ebola virus is an enveloped, negative-stranded RNA virus. This virus belongs to the family Filoviridae and has a distinctive morphology similar only to that of Marburg virus, but apparently has no antigenic characteristics of Marburg virus. Its genome is a nonsegmented RNA strand. SDS-PAGE profiles demonstrate the presence of seven structural proteins. These are the major nucleoprotein NP (104 K), glycoprotein GP (125K), L protein (180 K), and four proteins: VP40 (40 K), ‘V’PX (35 K), VP30 (30 K) and VP24 (24 K). All the proteins are encoded by monocistronic mRNA transcripts complementary to virion RNA. GP is the major protein of the surface spikes and only this 125K viral protein is glycosylated.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    Purity tested by electrophoresis.
  • 一次抗体 備考
    Ebola virus is an enveloped, negative-stranded RNA virus. This virus belongs to the family Filoviridae and has a distinctive morphology similar only to that of Marburg virus, but apparently has no antigenic characteristics of Marburg virus. Its genome is a nonsegmented RNA strand. SDS-PAGE profiles demonstrate the presence of seven structural proteins. These are the major nucleoprotein NP (104 K), glycoprotein GP (125K), L protein (180 K), and four proteins: VP40 (40 K), ‘V’PX (35 K), VP30 (30 K) and VP24 (24 K). All the proteins are encoded by monocistronic mRNA transcripts complementary to virion RNA. GP is the major protein of the surface spikes and only this 125K viral protein is glycosylated.
  • ポリ/モノ
    モノクローナル
  • クローン名
    FE37
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

プロトコール

参考文献

This product has been referenced in:
  • Sarrazy V  et al. Integrins avß5 and avß3 promote latent TGF-ß1 activation by human cardiac fibroblast contraction. Cardiovasc Res 102:407-17 (2014). Read more (PubMed: 24639195) »

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Sample
African Green Monkey Recombinant protein (Supernatant from VERO cells)
Gel Running Conditions
Reduced Denaturing (4-15% Bio-Rad TGX gel)
Loading amount
10 µg
Specification
Supernatant from VERO cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Aug 14 2015

Thank you for your enquiry. For the vial that you received, the concentration is 5.0 mg/ml. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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