製品の概要

  • 製品名Anti-Ebf4 antibody
    Ebf4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ebf4
  • 特異性This antibody regognizes Ebf4
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Cow, Dog, Human
  • 免疫原

    Synthetic peptide: VLAMYDRQGQ PVEVERTAFI DFVEKDREPG TEKTNNGIHY RLRLVYNNGL, corresponding to a region within N terminal amino acids 72-121 of Human Ebf4

  • ポジティブ・コントロール
    • SP2/0 lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 71 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay was 1:312500.

ターゲット情報

Anti-Ebf4 antibody 画像

  • Anti-Ebf4 antibody (ab49182) at 2.5 µg/ml + SP2/0 lysate at 10 µg with 5% skim milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 71 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

Anti-Ebf4 antibody (ab49182) 使用論文

ab49182 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab49182.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"