Anti-EBF1 抗体 [EPR4183] (ab108369)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4183] to EBF1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-EBF1 antibody [EPR4183]
EBF1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4183] to EBF1 -
由来種
Rabbit -
特異性
This antibody also recognises EBF3 protein.
-
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Raji, Daudi and 3T3-LI cell lysates. Mouse and rat spleen and lymph node tissue lysates.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4183 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108369の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/5000. Predicted molecular weight: 64 kDa.
For unpurified use at 1/10000. |
特記事項 |
---|
WB
1/5000. Predicted molecular weight: 64 kDa. For unpurified use at 1/10000. |
ターゲット情報
-
機能
Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. -
配列類似性
Belongs to the COE family.
Contains 1 IPT/TIG domain. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 1879 Human
- Entrez Gene: 13591 Mouse
- Entrez Gene: 116543 Rat
- Omim: 164343 Human
- SwissProt: Q9UH73 Human
- SwissProt: Q07802 Mouse
- SwissProt: Q63398 Rat
- Unigene: 573143 Human
see all -
別名
- COE1 antibody
- COE1_HUMAN antibody
- Collier, Olf and EBF transcription factor 1 antibody
see all
画像
-
All lanes : Anti-EBF1 antibody [EPR4183] (ab108369) at 1/1000 dilution (purified)
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Mouse lymph node lysate
Lane 5 : Rat spleen lysate
Lane 6 : Rat lymph node lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDaWe are unsure how to define the extra bands.
-
Anti-EBF1 antibody [EPR4183] (ab108369) at 1/1000 dilution (unpurifed) + Raji cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 20 µg
Predicted band size: 64 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-EBF1 antibody [EPR4183] (ab108369) at 1/1000 dilution (unpurified)
Lane 1 : Raji cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : 3T3-LI cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 64 kDa
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (8)
ab108369 は 8 報の論文で使用されています。
- Al Madhoun A et al. Caveolin-1 rs1997623 Single Nucleotide Polymorphism Creates a New Binding Site for the Early B-Cell Factor 1 That Instigates Adipose Tissue CAV1 Protein Overexpression. Cells 11:N/A (2022). PubMed: 36497195
- Park S et al. Thyroid Hormone Receptor a1 Mutants Impair B Lymphocyte Development in a Mouse Model. Thyroid 31:994-1002 (2021). PubMed: 33267733
- Yi E et al. Long noncoding RNA IL6-AS1 is highly expressed in chronic obstructive pulmonary disease and is associated with interleukin 6 by targeting miR-149-5p and early B-cell factor 1. Clin Transl Med 11:e479 (2021). PubMed: 34323408
- Chuang JH et al. Expression profiling of cell-intrinsic regulators in the process of differentiation of human iPSCs into retinal lineages. Stem Cell Res Ther 9:140 (2018). WB . PubMed: 29751772
- Xiao G et al. B-Cell-Specific Diversion of Glucose Carbon Utilization Reveals a Unique Vulnerability in B Cell Malignancies. Cell 173:470-484.e18 (2018). PubMed: 29551267
- Li J et al. Aryl Hydrocarbon Receptor Activation Suppresses EBF1 and PAX5 and Impairs Human B Lymphopoiesis. J Immunol 199:3504-3515 (2017). PubMed: 28978690
- Wang J et al. RNA-binding protein PSPC1 promotes the differentiation-dependent nuclear export of adipocyte RNAs. J Clin Invest 127:987-1004 (2017). PubMed: 28192372
- Woodcroft MW et al. Retrovirus-Mediated Expression of E2A-PBX1 Blocks Lymphoid Fate but Permits Retention of Myeloid Potential in Early Hematopoietic Progenitors. PLoS One 10:e0130495 (2015). PubMed: 26098938