The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: Antibody detection limit dilution 1:64,000.
WB: Use at a concentration of 1.0 - 3.0 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa). A minor band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunising peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.
The coiled coil domain is necessary for the homodimerization.
Golgi apparatus membrane. According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain.
Tskitishvili E et al. The effect of tumor-associated protein RCAS1 gene silencing on blood pressure and urinary protein excretion in pregnant mouse: a pilot study. Am J Obstet Gynecol203:364.e6-364.e12 (2010).
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