Anti-EB3 抗体 [KT36] (ab53360)
Key features and details
- Rat monoclonal [KT36] to EB3
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Human, African green monkey
- Isotype: IgG2a
製品の概要
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製品名
Anti-EB3 antibody [KT36]
EB3 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [KT36] to EB3 -
由来種
Rat -
アプリケーション
適用あり: ICC/IF, WB, Flow Cytmore details -
種交差性
交差種: Human, African green monkey -
免疫原
Full length human EB3 fused to a tag, purified from E. coli BL21 bacteria.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
KT36 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53360の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (3) |
1/100 - 1/1000.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
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Flow Cyt |
Use 1µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
1/100 - 1/1000. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
Flow Cyt
Use 1µg for 106 cells. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. -
組織特異性
Predominantly expressed in brain and muscle. -
配列類似性
Belongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain. -
ドメイン
Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1. -
細胞内局在
Cytoplasm > cytoskeleton. Associated with the microtubule network. - Information by UniProt
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参照データベース
- Entrez Gene: 22924 Human
- Omim: 605788 Human
- SwissProt: Q9UPY8 Human
- Unigene: 515860 Human
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別名
- APC binding protein antibody
- EB 3 antibody
- EB1 protein family member 3 antibody
see all
画像
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Anti-EB3 antibody [KT36] (ab53360) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 8 minutes -
Immunofluorescent staining for COS7 cells overexpressing EB3, using ab53360 at 1/100.
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Immunofluorescent staining of cell mitosis using ab53360 crude culture supernatant diluted 1:10 (Please note that there was about 50-100 ug/ml antibody in the supernatants) COS7 Transformed African Green Monkey Kidney Fibroblast Cells.
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Overlay histogram showing SH-SY5Y cells stained with ab53360 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53360, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab53360 は 10 報の論文で使用されています。
- Umair M et al. Biallelic variant in DACH1, encoding Dachshund Homolog 1, defines a novel candidate locus for recessive postaxial polydactyly type A. Genomics 113:2495-2502 (2021). PubMed: 34022343
- Palander O et al. Nonredundant roles of DIAPHs in primary ciliogenesis. J Biol Chem 296:100680 (2021). PubMed: 33872598
- Lagos-Cabré R et al. Ca2+ Release by IP3 Receptors Is Required to Orient the Mitotic Spindle. Cell Rep 33:108483 (2020). PubMed: 33326774
- Pavez M et al. STIM1 is required for remodelling of the endoplasmic reticulum and microtubule cytoskeleton in steering growth cones. J Neurosci N/A:N/A (2019). PubMed: 31023836
- Fassier C et al. Motor axon navigation relies on Fidgetin-like 1-driven microtubule plus end dynamics. J Cell Biol 217:1719-1738 (2018). PubMed: 29535193
- Drum BM et al. Oxidative stress decreases microtubule growth and stability in ventricular myocytes. J Mol Cell Cardiol 93:32-43 (2016). PubMed: 26902968
- Adam MG et al. SIAH ubiquitin ligases regulate breast cancer cell migration and invasion independent of the oxygen status. Cell Cycle 14:3734-47 (2015). WB . PubMed: 26654769
- Patel DM et al. Disease mutations in desmoplakin inhibit Cx43 membrane targeting mediated by desmoplakin-EB1 interactions. J Cell Biol 206:779-97 (2014). PubMed: 25225338
- Asanov A et al. A relay mechanism between EB1 and APC facilitate STIM1 puncta assembly at endoplasmic reticulum-plasma membrane junctions. Cell Calcium 54:246-56 (2013). PubMed: 23871111
- Bazellières E et al. Apico-basal elongation requires a drebrin-E-EB3 complex in columnar human epithelial cells. J Cell Sci 125:919-31 (2012). PubMed: 22275434