製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to EB3
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Horse, Cow, Dog
  • 免疫原

    Synthetic peptide:

    SGRLSNVAPPCILR

    , corresponding to internal sequence amino acids 172-185 of Human EB3 (NP_036458.2).

  • ポジティブ・コントロール
    • Human Brain (Cerebellum and Amygdala) and Mouse Brain lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab99287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 32 kDa.

ターゲット情報

  • 機能
    May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • 組織特異性
    Predominantly expressed in brain and muscle.
  • 配列類似性
    Belongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.
  • ドメイン
    Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
  • 細胞内局在
    Cytoplasm > cytoskeleton. Associated with the microtubule network.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APC binding protein antibody
    • EB 3 antibody
    • EB1 protein family member 3 antibody
    • EB3 antibody
    • EBF 3 antibody
    • EBF3 antibody
    • EBF3 S antibody
    • End binding protein 3 antibody
    • End-binding protein 3 antibody
    • MAPRE 3 antibody
    • MAPRE3 antibody
    • MARE3_HUMAN antibody
    • Member 3 antibody
    • Microtubule Associated Protein antibody
    • Microtubule associated protein RP/EB family member 3 antibody
    • Microtubule-associated protein RP/EB family member 3 antibody
    • OTTHUMP00000200910 antibody
    • RP 3 antibody
    • RP/EB Family antibody
    • RP3 antibody
    see all

Anti-EB3 antibody 画像

  • Anti-EB3 antibody (ab99287) at 0.3 µg/ml + Mouse brain lysates at 35 µg

    Predicted band size : 32 kDa
  • Anti-EB3 antibody (ab99287) at 0.1 µg/ml + Human brain (amygdala) lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 32 kDa
    Primary incubation was 1 hour.

プロトコール

Anti-EB3 antibody (ab99287) 使用論文

This product has been referenced in:
  • Sherman MA  et al. Soluble Conformers of Aß and Tau Alter Selective Proteins Governing Axonal Transport. J Neurosci 36:9647-58 (2016). Read more (PubMed: 27629715) »
  • Oz S  et al. The NAP motif of activity-dependent neuroprotective protein (ADNP) regulates dendritic spines through microtubule end binding proteins. Mol Psychiatry 19:1115-24 (2014). Read more (PubMed: 25178163) »

See all 2 Publications for this product

Product Wall

Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (N1E-115 mouse neuroblastoma cell line)
Total protein in input
1000 µg
Immuno-precipitation step
Protein A/G
Specification
N1E-115 mouse neuroblastoma cell line
Username

Mrs. Yanina Ivashko-Pachima

Verified customer

投稿 Aug 31 2017

DISCOUNT CODE: xxxx
Expiration date: xxxx

This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC and include this code in the “Additional Comments” section so...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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