製品の概要

  • 製品名Anti-EAPP antibody
    EAPP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EAPP
  • アプリケーション適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 32-142 of Human EAPP (Q56P03).

  • ポジティブ・コントロール
    • Human adrenal gland tissue; Human cell line U251 MG.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab121154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

ターゲット情報

  • 機能May play an important role in the fine-tuning of both major E2F1 activities, the regulation of the cell-cycle and the induction of apoptosis. Promotes S-phase entry, and inhibits p14(ARP) expression.
  • 組織特異性Ubiquitously expressed. Highest levels in heart, placenta, skeletal muscle and pancreas. Lower levels in brain, lung and kidney. In the brain, expressed in all regions with high levels in the cerebellum and cerebral cortex. Expressed in COS1 and transformed skin fibroblasts.
  • 発生段階During the cell cycle, expression disappears during mitosis.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1810011O16Rik antibody
    • BM-036 antibody
    • BM036 antibody
    • C14orf11 antibody
    • E2F-associated phosphoprotein antibody
    • EAPP antibody
    • EAPP_HUMAN antibody
    • RGD1309624 antibody
    see all

Anti-EAPP antibody 画像

  • ab121154 at 1/200 dilution staining EAPP in Paraffin-emebedded Human Adrenal gland tissue by Immunohistochemistry.
    Note: strong nuclear positivity in cortical cells.
  • ab121154 at 4 µg/ml staining EAPP in PFA/Triton X-100 fixed U251 MG cells by Immunofluorescence (green).
    Note; positivity in nucleus but not nucleoli and golgi apparatus.

Anti-EAPP antibody (ab121154) 使用論文

ab121154 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"