製品の概要

  • 製品名Anti-EAP30 antibody
    EAP30 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EAP30
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • 免疫原

    Synthetic peptide, derived from a region between amino acids 1-55 of Human EAP30.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab22768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4.
  • 配列類似性Belongs to the SNF8 family.
  • 細胞内局在Cytoplasm. Endosome membrane. Nucleus. Late endosome membrane. Recruited to the endosome membrane to participate in vesicle formation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dot3 antibody
    • ELL associated protein of 30 kDa antibody
    • ELL-associated protein of 30 kDa antibody
    • ESCRT-II complex subunit VPS22 antibody
    • hVps22 antibody
    • snf8 antibody
    • SNF8 ESCRTII complex subunit homolog (S cerevisiae) antibody
    • SNF8_HUMAN antibody
    • Vacuolar sorting protein SNF8 antibody
    • Vacuolar-sorting protein SNF8 antibody
    • VPS22 antibody
    see all

Anti-EAP30 antibody 画像

  • Anti-EAP30 antibody (ab22768) at 5 µg/ml + HepG2 Cell Lysate

    Secondary
    HRP conjugated anti-Rabbit IgG diluted in 1/50,000 - 100,000

    Performed under reducing conditions.

    Observed band size : 29 kDa (why is the actual band size different from the predicted?)
  • ab22768, at a 4.0-8.0 ug/ml dilution, staining human EAP30 in human stomach by immunohistochemistry, paraffin embedded tissue. Cells with positive label: epithelial cells of fundic gland (indicated with arrows) and surface mucous cells (indicated with arrow heads)
  • ab22768 staining EAP30 in Human heart tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).
  • ab22768 staining EAP30 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).

Anti-EAP30 antibody (ab22768) 使用論文

ab22768 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. You have followed the protocol recommended by our datasheet including the dilutions and blocking agent. Furthermore Eap30 expression has been ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"