製品の概要

  • 製品名Anti-EAF2 antibody
    EAF2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EAF2
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    AGFSHLDRRERV

    , corresponding to amino acids 5-16 of Human EAF2.

  • ポジティブ・コントロール
    • Rat and mouse liver for Western blot.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報IHC-P: Use at a concentration of 2.5 µg/ml.
    WB: Use at a concentration of 1.5 µg/ml. Predicted molecular weight: 29 kDa. This protein migrates to around 43kDa when a western blot is performed. We have found that the same happened in other literature as well. A paper published by Federico Simone shows that this protein can migrate to 43kDa (see references).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.
    • 組織特異性Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes.
    • 配列類似性Belongs to the EAF family.
    • 細胞内局在Nucleus speckle. Colocalizes with ELL to nuclear speckles.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BM 040 antibody
      • BM040 antibody
      • EAF 2 antibody
      • EAF2 antibody
      • EAF2_HUMAN antibody
      • Ehrlich S II transcriptional activator factor antibody
      • ELL associated factor 2 antibody
      • ELL-associated factor 2 antibody
      • Festa antibody
      • Testosterone regulated apoptosis inducer and tumor suppressor antibody
      • Testosterone regulated apoptosis inducer and tumor suppressor protein antibody
      • Testosterone-regulated apoptosis inducer and tumor suppressor protein antibody
      • TRAITS antibody
      • U19 antibody
      • Uncharacterized bone marrow protein BM 040 antibody
      • Uncharacterized bone marrow protein BM040 antibody
      see all

    Anti-EAF2 antibody (ab28357) 使用論文

    ab28357 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (293T cell lysate)
    Loading amount 30 µg
    Specification 293T cell lysate
    Treatment Transfected with EAF2
    Gel Running Conditions Non-reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. Laura Pascal

    Verified customer

    投稿 May 06 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"