The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 2.5 µg/ml.
WB: Use at a concentration of 1.5 µg/ml. Predicted molecular weight: 29 kDa. This protein migrates to around 43kDa when a western blot is performed. We have found that the same happened in other literature as well. A paper published by Federico Simone shows that this protein can migrate to 43kDa (see references).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.
Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes.
Belongs to the EAF family.
Nucleus speckle. Colocalizes with ELL to nuclear speckles.