製品の概要

  • 製品名Anti-EAAT2 antibody
    EAAT2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to EAAT2
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cat, Dog, Orangutan
  • 免疫原

    Recombinant fragment: DEVSSLDAFL DLIRNLFPEN LVQACFQQIQ TVTKKVLVAP PPDEEANATS AVVSLLNETV TEVPEETKMV IKKGLEFKDG, corresponding to amino acids 160 - 239 of Human EAAT2 (NP_004162) with a 26 kDa tag

  • ポジティブ・コントロール
    • HeLa cell lysate; immunogen This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cerebral cortex.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab77039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 62 kDa.
ELISA Use at an assay dependent concentration. Detection limit for recombinant tagged EAAT2 is approximately 0.03ng/ml.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
  • 配列類似性Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
  • 翻訳後修飾Glycosylated.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EAA2_HUMAN antibody
    • EAAT2 antibody
    • Excitatory amino acid transporter 2 antibody
    • Excitotoxic amino acid transporter 2 antibody
    • Glial high affinity glutamate transporter antibody
    • GLT 1 antibody
    • GLT1 antibody
    • Glutamate aspartate transporter II antibody
    • Glutamate transporter 1 antibody
    • Glutamate/aspartate transporter II antibody
    • Slc1a2 antibody
    • Sodium dependent glutamate aspartate transporter 2 antibody
    • Sodium-dependent glutamate/aspartate transporter 2 antibody
    • solute carrier family 1 (glial high affinity glutamate transporter), member 2 antibody
    • Solute carrier family 1 glial high affinity glutamate transporter member 2 antibody
    • Solute carrier family 1 member 2 antibody
    see all

Anti-EAAT2 antibody 画像

  • IHC image of EAAT2 staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77039, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-EAAT2 antibody (ab77039) at 1/500 dilution + recombinant tagged EAAT2 (the immunogen) at 0.2 µg/ml

    Secondary
    goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 62 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Anti-EAAT2 antibody (ab77039) at 1/500 dilution + HeLa cell lysate expressing EAAT2 at 25 µg

    Secondary
    goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 62 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,50 kDa. We are unsure as to the identity of these extra bands.
  • Capture ELISA using ab77039 and recombinant tagged EAAT2

Anti-EAAT2 antibody (ab77039) 使用論文

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"