製品の概要

  • 製品名Anti-EAAT2 antibody
    EAAT2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EAAT2
  • アプリケーション適用あり: IHC-FoFr, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Rat Glutamate Transporter 1 (EAAT2).

    (Peptide available as ab41752.)

  • ポジティブ・コントロール
    • ab41621 gave a positive signal in both Mouse and Rat Brain Tissue Lysates and in PC12 cell line

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab41621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
  • 配列類似性Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
  • 翻訳後修飾Glycosylated.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EAA2_HUMAN antibody
    • EAAT2 antibody
    • Excitatory amino acid transporter 2 antibody
    • Excitotoxic amino acid transporter 2 antibody
    • Glial high affinity glutamate transporter antibody
    • GLT 1 antibody
    • GLT1 antibody
    • Glutamate aspartate transporter II antibody
    • Glutamate transporter 1 antibody
    • Glutamate/aspartate transporter II antibody
    • Slc1a2 antibody
    • Sodium dependent glutamate aspartate transporter 2 antibody
    • Sodium-dependent glutamate/aspartate transporter 2 antibody
    • solute carrier family 1 (glial high affinity glutamate transporter), member 2 antibody
    • Solute carrier family 1 glial high affinity glutamate transporter member 2 antibody
    • Solute carrier family 1 member 2 antibody
    see all

Anti-EAAT2 antibody 画像

  • All lanes : Anti-EAAT2 antibody (ab41621) at 1 µg/ml

    Lane 1 : Brain (Mouse) Mouse Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
  • Immunohistochemical staining of PFA-fixed, frozen mouse brain tissue using undiluted ab41621. Tissue sections were permeabilized using Triton-X100 and incubated with ab41621 for 12 hours at 4°C. ab150077 (goat anti-rabbit IgG H&L Alexa Fluor® 488) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab41621 stained pc12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41621, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-EAAT2 antibody (ab41621) 使用論文

This product has been referenced in:
  • Beaudet MJ  et al. High yield extraction of pure spinal motor neurons, astrocytes and microglia from single embryo and adult mouse spinal cord. Sci Rep 5:16763 (2015). Read more (PubMed: 26577180) »
  • Sohn J  et al. The subventricular zone continues to generate corpus callosum and rostral migratory stream astroglia in normal adult mice. J Neurosci 35:3756-63 (2015). IHC-FoFr ; Mouse . Read more (PubMed: 25740506) »

See all 9 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Spinal cord)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 20 µg
Specification Spinal cord
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jul 29 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain tissue)
Permeabilization Yes - Triton-X100
Specification Brain tissue
Fixative Paraformaldehyde
Username

Dr. Daniel Berg

Verified customer

投稿 Aug 20 2015

Thank you for contacting us.
I have contacted the Abreview customer regarding more information about the staining with ab41621. I will let you know if I hear back from her.
As for ab61859, I have the following IHC-P protocol:
General IHC...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

投稿 Jan 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"