Application
Western blot
Sample
Mouse Cell lysate - whole cell (lung endothelial cells)
Gel Running Conditions
Reduced Denaturing (gradient gel)
Loading amount
10 µg
Specification
lung endothelial cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/500
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: IRDye680LT Anti-Rabbit (LICOR)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Detection
Detection method
Fluorescence
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 110 kDa Non-specific: 150 kDa
Additional data
Additional Notes
First Lane: Ladder
Second Lane: Cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 12 2013