Application
Western blot
Sample
Rat Tissue lysate - whole (skeletal muscle (plantaris))
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
40 µg
Specification
skeletal muscle (plantaris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% TBST-milk
Dilution
1/625
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Peroxidase AffiniPure Donkey Anti-Mouse IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
-
Exposure
8 minute(s) and 0 second(s)
Bands
Specific: ~133 kDa Non-specific: - kDa
Positive control
-
Additional data
Additional Notes
PVDF membrane - 0.45 um pore size
6 animals from control group, 20 animals from experimental groups
Abcam response
Thank you for participating in our Abtrial program and your valuable feedback. We are pleased that this antibody worked well for you in rat, a species that has not been validated yet. We welcome any further feedback and Abreviews from other customers who have used this antibody in rat which will help us assess how well the antibody works in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 04 2015