Application
Western blot
Sample
Human Tissue lysate - whole (Skeletal muscle)
Loading amount
20 µg
Specification
Skeletal muscle
Gel Running Conditions
Reduced Denaturing (8% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% milk in TBS-Tween (0.005%)
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Mouse IgG, HRP-linked Antibody
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
20 second(s)
Bands
Specific: 133 kDa
Additional data
Additional Notes
Nitrocellulose membrane;
1% SDS lysis buffer with 1:100 protease inhibitors.
1% SDS lysis buffer with 1:100 protease inhibitors.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. G.K Sakellariou
Verified customer
投稿 May 15 2013