Application
Western blot
Sample
Human Cell lysate - whole cell (Nasal epithelial cells, bronchial epithelial cells)
Gel Running Conditions
Non-reduced Denaturing (6%)
Loading amount
20 µg
Specification
Nasal epithelial cells, bronchial epithelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-rabbit IgG Antibody
Host species: Human
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Human
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 220 kDa
Positive control
Nasal epithelial cells (from tissue), BEAS-2B cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Kyeongah Kim
Verified customer
投稿 Jun 16 2017