Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
15 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: LI-COR goat anti rabbit 800CW
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Dilution
1/15000
Detection
Detection method
LI-COR odyssey scanner
Exposure
8 minute(s) and 0 second(s)
Bands
Specific: 47 kDa Non-specific: 120 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 07 2011