Anti-EIF2B5 抗体 (ab91565)
Key features and details
- Rabbit polyclonal to EIF2B5
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-EIF2B5 antibody
EIF2B5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EIF2B5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human EIF2B5 aa 400-450.
Database link: NP_003898.2 -
ポジティブ・コントロール
- HeLa and 293T whole cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab91565 was affinity purified using an epitope specific to EIF2B5 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab91565の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 80 kDa.
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IP |
Use at 5-15 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 80 kDa. |
IP
Use at 5-15 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. -
関連疾患
Defects in EIF2B5 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy. -
配列類似性
Belongs to the eIF-2B gamma/epsilon subunits family.
Contains 1 W2 domain. - Information by UniProt
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参照データベース
- Entrez Gene: 8893 Human
- Omim: 603945 Human
- SwissProt: Q13144 Human
- Unigene: 283551 Human
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別名
- CACH antibody
- CLE antibody
- EI2BE_HUMAN antibody
see all
画像
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All lanes : Anti-EIF2B5 antibody (ab91565) at 0.1 µg/ml
Lanes 1 & 4 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 80 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling EIF2B5 with ab91565 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of EIF2B5 by Western Blot of Immunprecipitate.
ab91565, at 1 µg/ml, staining EIF2B5 in HeLa whole cell lysate immunoprecipitated using ab91565 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab91565 は論文での使用が確認できていません。