製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to E4F1
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to a region between residue 275 and 325 of human E4F1

  • ポジティブ・コントロール
    • HeLa, 293T, and MCF7 whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab70615 was affinity purified using an epitope specific to E4F1 immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab70615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 84 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      E4F1 is a zinc finger transcription factor that inhibits cell proliferation. E4F1 interacts with Bmi1 in hematopoietic cells and is important for the regulation of stem cell proliferation.
    • 細胞内局在
      Nuclear
    • 参照データベース
    • 別名
      • E4F antibody
      • E4F transcription factor 1 antibody
      • E4F transcription factor 1, isoform CRA_b antibody
      • p120E4F antibody
      • P120E4F transcription factor antibody
      • p50E4F antibody
      • Putative E3 ubiquitin-protein ligase E4F1 antibody
      • RING type E3 ubiquitin transferase E4F1 antibody
      • Transcription factor E4F1 antibody
      see all

    画像

    • All lanes : Anti-E4F1 antibody (ab70615) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : 293T whole cell lysate
      Lane 3 : MCF7 whole cell lysate

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 84 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes
    • Detection of Human E4F1 by Western Blot of Immunprecipitate. ab70615 at 1µg/ml staining E4F1 in MCF7 whole cell lysates immunoprecipitated using ab70615 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 minutes

    参考文献

    ab70615 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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