Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession.
Belongs to the E2F/DP family.
Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer.
All lanes : Anti-E2F8 antibody (ab109596) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 5 µg Lane 4 : HeLa whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 94 kDa
Exposure time : 3 minutes
Immunoprecipitation - E2F8 antibody (ab109596)
Detection of E2F8 by Western Blot of Immunprecipitate.
ab109596 at 1µg/ml staining E2F8 in HeLa whole cell lysate immunoprecipitated using ab109596 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds