製品の概要

  • 製品名Anti-E2F7 antibody
    E2F7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to E2F7
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Horse, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide (Mouse)

  • ポジティブ・コントロール
    • SP2/0 cell lysate and mouse stomach tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab56022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能Along with E2F8, inhibitor of E2F-dependent transcription that is important for the control of the E2F1-TP53 apoptotic pathway. Directly represses E2F1 transcription (By similarity). Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession.
  • 配列類似性Belongs to the E2F/DP family.
  • ドメインBoth DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 製品の状態There are 2 isoforms produced by alternative splicing.
  • 別名
    • E2F 7 antibody
    • E2F transcription factor 7 antibody
    • E2F-7 antibody
    • E2f7 antibody
    • E2F7_HUMAN antibody
    • FLJ12981 antibody
    • Transcription factor E2F7 antibody
    see all

Anti-E2F7 antibody 画像

  • Anti-E2F7 antibody (ab56022) at 0.5 µg/ml + SP2/0 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 99 kDa
    Observed band size : 99 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue labelling E2F7 with ab56022 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the fundic gland. Magnification: 400X.

Anti-E2F7 antibody (ab56022) 使用論文

This product has been referenced in:
  • Yin W  et al. Elevated E2F7 expression predicts poor prognosis in human patients with gliomas. J Clin Neurosci 33:187-193 (2016). Read more (PubMed: 27460513) »
  • Tan JY  et al. Identification of Cellular Targets of MicroRNA-181a in HepG2 Cells: A New Approach for Functional Analysis of MicroRNAs. PLoS One 10:e0123167 (2015). WB ; Human . Read more (PubMed: 25901570) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (1wk old Liver)
Loading amount 40 µg
Specification 1wk old Liver
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
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投稿 Dec 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"