製品の概要
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製品名Anti-E2F7 antibody
E2F7 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to E2F7
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由来種Goat
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アプリケーション適用あり: WBmore details
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種交差性交差種: Human
交差が予測される動物種: Cow, Dog, Pig
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免疫原
Synthetic peptide:
C-DLSKQKKFTPERNP
, corresponding to amino acids 53-66 of Human E2F7 (NP_976328.2). -
ポジティブ･コントロール
- Human kidney, testis, heart and brain cerebellum lysates
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab106832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa). |
ターゲット情報
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機能Along with E2F8, inhibitor of E2F-dependent transcription that is important for the control of the E2F1-TP53 apoptotic pathway. Directly represses E2F1 transcription (By similarity). Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession.
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配列類似性Belongs to the E2F/DP family.
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ドメインBoth DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 144455 Human
- Omim: 612046 Human
- SwissProt: E1BE02 Cow
- SwissProt: Q96AV8 Human
- Unigene: 416375 Human
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製品の状態There are 2 isoforms produced by alternative splicing.
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別名
- E2F 7 antibody
- E2F transcription factor 7 antibody
- E2F-7 antibody
see all
画像
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Western blot - Anti-E2F7 antibody (ab106832)Anti-E2F7 antibody (ab106832) at 0.1 µg/ml + Human kidney lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 1 hour
プロトコール
参考文献
ab106832 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"