製品の概要

  • 製品名
    Anti-E2F6 antibody [TFE61]
    E2F6 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [TFE61] to E2F6
  • アプリケーション
    適用あり: IP, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human)

  • ポジティブ・コントロール
    • Hela cells or K-562 nuclear extract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP
ICC/IF
WB
  • 追加情報
    WB: 1/500 - 1/1000. Detects a band of approximately 38 kDa from K-562 nuclear extract(predicted molecular weight: 32 kDa). For optimal results, primary antibody incubations should be performed at 37°C

    ICC/IF: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
    • 組織特異性
      Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
    • 配列類似性
      Belongs to the E2F/DP family.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • E2F 6 antibody
      • E2F binding site modulating activity protein antibody
      • E2F transcription factor 6 antibody
      • E2F transcription factor 6 isoform 1 antibody
      • E2F-6 antibody
      • E2F6 antibody
      • E2F6_HUMAN antibody
      • EMA antibody
      • MGC111545 antibody
      • Transcription factor E2F6 antibody
      see all

    画像



    • Predicted band size : 32 kDa


      Detection of E2F6 by Western blot analysis. E2F6 is detected in nuclear extracts derived from K-562 cells using a 1/1000 dilution of ab11952.

    参考文献

    This product has been referenced in:
    • Hansen HG  et al. Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells. Sci Rep 5:18016 (2015). Read more (PubMed: 26657798) »
    • Furuta M  et al. miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma. Carcinogenesis 31:766-76 (2010). WB ; Human . Read more (PubMed: 19843643) »

    See all 3 Publications for this product

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