製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to E2F3
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: LLQQTEDQIP SNLEGPFVNL LPPLLQEDYL LSLGEEEGIS DLFDAYDLEK, corresponding to amino acids 406-455 of Human E2F3

  • ポジティブ・コントロール
    • Fetal thymus lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 21297112
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ターゲット情報

画像

  • Anti-E2F3 antibody (ab50917) at 0.25 µg/ml + Fetal thymus lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab50917 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50917, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab50917 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab50917, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献

This product has been referenced in:
  • Qiu M  et al. MicroRNA-200c suppresses cell growth and metastasis by targeting Bmi-1 and E2F3 in renal cancer cells. Exp Ther Med 13:1329-1336 (2017). WB ; Human . Read more (PubMed: 28413473) »
  • Guo Y  et al. miR-564 is downregulated in gastric carcinoma and targets E2F3. Oncol Lett 13:4155-4160 (2017). Read more (PubMed: 28588702) »

See all 7 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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