製品の概要
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製品名Anti-E2F1 (phospho S364) antibody [2]
E2F1 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [2] to E2F1 (phospho S364)
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由来種Mouse
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アプリケーション適用あり: WB, IPmore details
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種交差性交差種: Human
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免疫原
A synthetic peptide corresponding to a sequence of Human E2F1 protein including and surrounding phospho-Ser364
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ポジティブ･コントロール
- MCF cells treated with etoposide at 10 µM for 16 h
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
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バッファーConstituents: 49% PBS, 50% Glycerol
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Concentration information loading... -
精製度Ion Exchange Chromatography
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特記事項（精製）Produced in serum-free medium and purified under mild conditions
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ポリ/モノモノクローナル
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クローン名2
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アイソタイプIgG2b
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軽鎖の種類kappa
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab122888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. | |
| IP | Use at an assay dependent concentration. |
ターゲット情報
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機能Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
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配列類似性Belongs to the E2F/DP family.
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翻訳後修飾Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. -
細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 1869 Human
- Omim: 189971 Human
- SwissProt: Q01094 Human
- Unigene: 654393 Human
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別名
- Dmel\CG6376 antibody
- Dmel_CG6376 antibody
- drosE2F1 antibody
see all
画像
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![Western blot - Anti-E2F1 (phospho S364) antibody [2] (ab122888)](http://a.abcam.com/ps/datasheet/images/122/ab122888/E2F1-Primary-antibodies-ab122888-1.jpg)
Western blot - Anti-E2F1 (phospho S364) antibody [2] (ab122888)All lanes : Anti-E2F1 (phospho S364) antibody [2] (ab122888) at 1 µg/ml
Lane 1 : Untreated MCF cells immunoprecipitated with a pantropic anti-E2F1 antibody
Lane 2 : MCF cells treated with etoposide at 10 µM for 16 h, immunoprecipitated with a pantropic anti-E2F1 antibody
Lane 3 : MCF cells treated with etoposide at 10 µM for 16 h
Predicted band size: 47 kDa
参考文献
ab122888 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"