Anti-E2F1 (phospho S364) 抗体 [2] (ab122888)

製品の概要

• 製品名
  Anti-E2F1 (phospho S364) antibody [2]
  E2F1 一次抗体 製品一覧
• 製品の詳細
  Mouse monoclonal [2] to E2F1 (phospho S364)
• 特異性
  ab122888 is specific to the Human E2F1 protein phosphorylated at Ser364.
• アプリケーション
  適用あり: WB, IPmore details
• 種交差性
  交差種: Human
  
• 免疫原

  A synthetic peptide corresponding to a sequence of Human E2F1 protein including and surrounding phospho-Ser364

• ポジティブ･コントロール
  • MCF cells treated with etoposide at 10 µM for 16 h

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
• バッファー
  Constituents: 49% PBS, 50% Glycerol
• Concentration information loading...
• 精製度
  IgG fraction
• 特記事項（精製）
  Produced in serum-free medium and purified under mild conditions
• ポリ/モノ
  モノクローナル
• クローン名
  2
• アイソタイプ
  IgG2b
• 軽鎖の種類
  kappa
• 研究分野
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Forkhead Box

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • Rb family

関連製品

• Compatible Secondaries
  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

アプリケーション

ターゲット情報

• 機能
  Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
• 配列類似性
  Belongs to the E2F/DP family.
• 翻訳後修飾
  Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
  Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
• 細胞内局在
  Nucleus.

• Target information above from: UniProt accession Q01094 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 1869 Human
  • Omim: 189971 Human
  • SwissProt: Q01094 Human
  • Unigene: 654393 Human
• 別名
  • Dmel\CG6376 antibody
  • Dmel_CG6376 antibody
  • drosE2F1 antibody

  • E(Sev-CycE)3A antibody
  • E(var)3-93E antibody
  • E2-promoter binding facto antibody
  • E2F 1 antibody
  • E2F transcription factor 1 antibody
  • E2F-1 antibody
  • E2f-PA antibody
  • E2f-PB antibody
  • E2f-PC antibody
  • E2F1 antibody
  • E2f1 E2F transcription factor 1 antibody
  • E2F1_HUMAN antibody
  • Evar(3)164 antibody
  • KIAA4009 antibody
  • l(3)07172 antibody
  • l(3)j3B1 antibody
  • l(3)j3C2 antibody
  • l(3)rM729 antibody
  • mKIAA4009 antibody
  • OTTHUMP00000030661 antibody
  • PBR3 antibody
  • PRB binding protein E2F 1 antibody
  • PRB-binding protein E2F-1 antibody
  • RBAP 1 antibody
  • RBAP-1 antibody
  • RBAP1 antibody
  • RBBP-3 antibody
  • RBBP3 antibody
  • RBP 3 antibody
  • RBP3 antibody
  • Retinoblastoma-associated protein 1 antibody
  • Retinoblastoma-binding protein 3 antibody
  • Transcription factor E2F1 antibody

  see all

プロトコール

• Western blot protocols
• Immunoprecipitation protocols