Anti-E2F1 (phospho S364) 抗体 [2] (ab122888)
Key features and details
- Mouse monoclonal [2] to E2F1 (phospho S364)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-E2F1 (phospho S364) antibody [2]
E2F1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2] to E2F1 (phospho S364) -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human E2F1. A synthetic peptide corresponding to a sequence of Human E2F1 protein including and surrounding phospho-Ser364
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ポジティブ・コントロール
- MCF cells treated with etoposide at 10 µM for 16 h
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
バッファー
pH: 6
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Ion Exchange Chromatography -
特記事項(精製)
Produced in serum-free medium and purified under mild conditions -
ポリ/モノ
モノクローナル -
クローン名
2 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122888の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. |
ターゲット情報
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機能
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. -
配列類似性
Belongs to the E2F/DP family. -
翻訳後修飾
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1869 Human
- Omim: 189971 Human
- SwissProt: Q01094 Human
- Unigene: 654393 Human
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別名
- Dmel\CG6376 antibody
- Dmel_CG6376 antibody
- drosE2F1 antibody
see all
画像
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All lanes : Anti-E2F1 (phospho S364) antibody [2] (ab122888) at 1 µg/ml
Lane 1 : Untreated MCF cells immunoprecipitated with a pantropic anti-E2F1 antibody
Lane 2 : MCF cells treated with etoposide at 10 µM for 16 h, immunoprecipitated with a pantropic anti-E2F1 antibody
Lane 3 : MCF cells treated with etoposide at 10 µM for 16 h
Predicted band size: 47 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab122888 は論文での使用が確認できていません。