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Product name
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate
E. coli Positive Control 抗体 (2件) 一覧
General notes
This E.coli lysate contains a 41 kd recombinant protein expressing 11 commonly used Tags which are fused to the N-terminus of the protein in the order of:
T7 HSV
C-Myc
VSV-g
Glu-Glu
V5
E-tag
FLAG (DDDDK)
S-tag
HA
6x His
Tag sequences: T7 = CMASMTGGQQMG
HSV = QPELAPEDPEDC
CMYC = EQKLISEEDLC
VSV-g = YTDIEMNRLGK
GluGlu = EYMPME
V5 = GKPIPNPLLGLDST
E-tag = GAPVPYPDPLEPR
FLAG = DYKDDDDK
HA = YPYDVPDYAC
6xHIS = HHHHHH
T7 HSV
C-Myc
VSV-g
Glu-Glu
V5
E-tag
FLAG (DDDDK)
S-tag
HA
6x His
Tag sequences: T7 = CMASMTGGQQMG
HSV = QPELAPEDPEDC
CMYC = EQKLISEEDLC
VSV-g = YTDIEMNRLGK
GluGlu = EYMPME
V5 = GKPIPNPLLGLDST
E-tag = GAPVPYPDPLEPR
FLAG = DYKDDDDK
HA = YPYDVPDYAC
6xHIS = HHHHHH
Tested applications
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration
Concentration information loading...
Purity
Whole Cell Lysate
Purification Notes
E. coli were lysed with SDS-PAGE sample loading dye, boiled at 95°C for 10 minutes, then centrifuged at 13,000 g for 15 minutes.
ライゼート notes
Excellent as a loading control for Western blotting. Lysate expresses 11 different epitope tags.
Research areas
Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Arabidopsis / Miscellaneous
Tags & Cell Markers >> Epitope Tags >> Other Tags >> HSV Tag
Tags & Cell Markers >> Epitope Tags >> Other Tags >> S Tag
Tags & Cell Markers >> Epitope Tags >> Other Tags >> VSV-G Tag
Tags & Cell Markers >> Epitope Tags >> Other Tags >> E tag
Microbiology >> Organism >> Bacteria >> Gram negative Bacteria >> Escherichia coli
Tags & Cell Markers >> Epitope Tags >> T7 Tag
Tags & Cell Markers >> Epitope Tags >> V5 Tag
Tags & Cell Markers >> Epitope Tags >> Glu-Glu Tag
Tags & Cell Markers >> Epitope Tags >> DDDDK Tag
Tags & Cell Markers >> Epitope Tags >> Myc Tag
Tags & Cell Markers >> Epitope Tags >> HA Tag
Background
Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Use 0.5 - 2.0 µl for 10 - 12% gels.
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate images:
Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
All lanes : Anti-6X His tag® antibody - ChIP Grade (ab9108) at 1/1000 dilution
Lane 1 :
Lane 2 :
Lane 3 :
Lane 4 :
Western blot
Anti-Myc tag antibody (ab107247) at 1 µg/ml +
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 41 kDa (why is the actual band size different from the predicted?)
This WB was performed using an E. coli lysate that expresses a 41kDa recombinant protein comprised of 11 commonly used epitope tags (T7, HSV, C-Myc, VSV-g, Glu-Glu, V5, E-tag, FLAG, S-tag, HA and 6xHis). This lysate is available as ab5395.
References for E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
This product has been referenced in:
- Miriam_Sans, Itzel_Vargas-Prez, Luis_Quintales, Fr Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe. Nucleic Acids Res : 1-11 (2011). WB; Schizosaccharomyces pombe.Read more (PubMed: 21515633) »
See 1 publication for this product
Publishing research using ab5395? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"