E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431)

製品の概要

  • 製品名E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein
    E. coli Positive Control ライゼート 製品一覧
  • 特記事項


    This lysate can be used as a positive control for Western blots using lysates containing 6X-His tagged proteins.

  • アプリケーション適用あり: WBmore details

製品の特性

  • 製品の状態Lyophilised:After the lyophilised pellet is dissolved in 250 ul SDS-gel sample buffer (1 mg/ml protein lysate) it can be used direct for sample loading and then stored at –20°C. The sample can be reused several times
  • 保存方法Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • ライゼート 備考

    The cell lysate was prepared from E.coli (Tuner [De3]) expressing a 14.2 kDa protein containing a C-terminal 6xHis tag.

  • 研究分野
  • 背景Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.

アプリケーション

Our Abpromise guarantee covers the use of ab2431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. This E. coli (strain BL21)lysate contains a recombinant C-terminal 6x-His tagged protein of 17 kDa. The his-tagged protein is monomeric and will be detected under reducing and non-reducing conditions. Use 5-20 µg per lane of a mini-gel at 12-15 % SDS-PAGE. After the lyophilised pellet is dissolved in 250 ul SDS-gel sample buffer (1 mg/ml protein lysate) it can be used direct for sample loading and than stored at -20°C. The sample can be reused several times.

E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein 画像

  • Western blot of 9 µg of E. coli BL21 lysate containing a 17kDa 6x-His tagged protein.  Mouse monoclonal to His tag (4D11 - ab5000) is used at 1:1000.

E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) 使用論文

This product has been referenced in:
  • Wright JC  et al. Enhanced peptide identification by electron transfer dissociation using an improved mascot percolator. Mol Cell Proteomics : (2012). Read more (PubMed: 22493177) »

See 1 Publication for this product

Product Wall

E coli was grown in LB-Amp medium.

The E. coli was cultured using a standard protocol and harvested by centrifugation. The pellet was resuspended in 50mM Tris, 10mM EDTA, pH8.0, treated with ultrasonic sound, centrifuged and the supernatant was finally filtered (0.22µm).
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Merci de votre émail.

Nous sommes désolés que le produit ab2431 ne contenait pasla quantitéattendu. Je me permets de vous transférer le numéro de commande de remplacement gratuit de ce produit : xx. ...

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Merci beaucoup pour votre patience.

Le laboratoire a retesté le même lot d'ab2431 sur SDS PAGE et coomassie- veuillez voir le résultat en document attaché. Le lysat était reconstitué en 250µl 1X ...

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Thank you for kindly confirming these details.

As requested, we would be pleased to arrange this free of charge replacement. In order to do this, I am forwarding these details on to our distributors team who will be happy to organize this fo...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to ...

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Merci pour votre appel ainsi que votre patience.

Je peux vous confirmer que les cellules E. coli du produit ab2431 ont été préparé dans un tampon contenant 50mM Tris, 10mM EDTA, pH 8.0 et lysé par sonification ensuite. Le lysat était ensuite...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this lysate.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee, In the e...

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Thank you for your inquiry. We use the following 4X sample buffer: 4 mL mQ H2O 1 mL 0.5M Tris-Cl, pH 6.8 0.8 mL Glycerol 1.6 mL 10% SDS (w/v) 0.2 mL Bromphenol blue + 400µl Mercaptoethanol (for reduced gels) If you reconstitu...

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The E. coli lysate containing the his-tag protein is a whole bacterial cell pellet lysate. The amount of bands detected with a his-tag antibody depends on the quality of the his-tag antibody and is therefore antibody dependent.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"