製品の概要

  • 製品名Anti-E. coli O157 antibody [3011]
    E. coli O157 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3011] to E. coli O157
  • 特異性Specific for E. coli serotype O157. The region recognized by this antibody is LPS oligosaccharide. Non-reactive with serotypes: O111, O125, O20 and O55 and K12.
  • アプリケーション適用あり: IP, ICC/IF, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Escherichia coli
  • 免疫原

    E. coli 0157 whole cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 10mM PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)>90% pure.
  • ポリ/モノモノクローナル
  • クローン名3011
  • ミエローマunknown
  • アイソタイプIgG3
  • 軽鎖の種類unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab20976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent dilution. Immunoprecipitates LPS of O157:H7. May be conjugated to colloidal gold.
ICC/IF Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution. May be used as either a capture or detection antibody.
IHC-P Use at an assay dependent concentration. PubMed: 21858177

ターゲット情報

  • 関連性Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.
  • 別名
    • Escherichia coli O157 antibody

Anti-E. coli O157 antibody [3011] (ab20976) 使用論文

This product has been referenced in:
  • Etienne-Mesmin L  et al. Interactions with M Cells and Macrophages as Key Steps in the Pathogenesis of Enterohemorragic Escherichia coli Infections. PLoS One 6:e23594 (2011). IHC-P . Read more (PubMed: 21858177) »
  • Park S  et al. Enzyme-linked immuno-strip biosensor to detect Escherichia coli O157:H7. Ultramicroscopy 108:1348-51 (2008). Read more (PubMed: 18562109) »

See all 3 Publications for this product

Product Wall

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We would not be able to confirm the DH5alpha specifictiy of ab37204 and ab20976. However we can confirm ab25823 and ab48416 would work with DH5alpha species.

I hope this information is helpful to you. Plea...

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I'm happy to help. Please let me know if you have any further questions.

Thank you for your enquiry. We typically do not have IEF data for our antibodies. I have found this reference for you that might be of some help: http://onlinelibrary.wiley.com/doi/10.1002/elps.1150060305/abstract Please let me know if you need anyth...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"