製品の概要

  • 製品名Anti-E. coli O152 antibody
  • 製品の詳細
    Rabbit polyclonal to E. coli O152
  • 特異性Specific to E. coli somatic O152 antigen
  • アプリケーション適用あり: Agglutination, ICC/IFmore details
  • 種交差性
    Reacts with E. coli O152.
  • 免疫原

    Tissue/ cell preparation of E. coli O152 (heat inactivated).

  • ポジティブ・コントロール
    • Antigenic suspension.
  • 特記事項Reagents should be allowed to stand at 15°C - 25°C for at least 30 minutes prior to use.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.08% Sodium Azide
    Constituents: Whole serum
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab78978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Agglutination Use at an assay dependent concentration.

See attached protocol. Use heat inactivated bacteria.

ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性Escherichia coli are a group of gram-negative bacilli belonging to the family Enterobacteriaceae and is considered as one of the bacteria in the human intesinal normal microbial flora. Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigen (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli. These are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E.coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requries verification of their pathogenicity, pathogenic E. coli have often specific serotypes; therefore, typing of the serogroup and serotype is necessary to screening pathogenic E. coli.

Anti-E. coli O152 antibody 画像

  • ab78978 staining E.coli in mouse colon tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Sections were snap frozen without fixation and incubated in 1% PFA in PBS after cutting sections (5µm). Sections were permeabilized with 0.5% NP40 and blocked with 10% goat serum for 1 hour at 25°C. Sections were incubated with primary antibody (1/500 in 5% goat serum) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

Anti-E. coli O152 antibody (ab78978) 使用論文

ab78978 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Cell (mouse colon sections (snap frozen))
Specification mouse colon sections (snap frozen)
Permeabilization Yes - 0.5% NP40
Fixative snap frozen without fixation 1% PFA in PBS after cutting sections
Username

Dr. Jerry Turner

Verified customer

投稿 Feb 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"