製品の概要

  • 製品名
    Anti-E. coli antibody (FITC)
    E. coli 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to E. coli (FITC)
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • アプリケーション
    適用あり: ICC/IFmore details
  • 種交差性
    交差種: Escherichia coli
  • 免疫原

    Tissue/ cell preparation (Escherichia coli, all serotypes). A mixed culture of E. coli strains (O157:H7, O20, O125, O55, O111 & K12), intact plus lysed, non-denatured, was gamma-inactivated and used directly as the immunogen.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: 40% Glycerol, 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab30522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/25. Acetone fixation of the antigen is recommended.

ターゲット情報

  • 関連性
    Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
  • 別名
    • Escherichia coli antibody

Anti-E. coli antibody (FITC) (ab30522) 使用論文

This product has been referenced in:
  • Pazos-Perez N  et al. Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids. Sci Rep 6:29014 (2016). Read more (PubMed: 27364357) »
  • Golberg A  et al. Cloud-enabled microscopy and droplet microfluidic platform for specific detection of Escherichia coli in water. PLoS One 9:e86341 (2014). Read more (PubMed: 24475107) »

See all 3 Publications for this product

Product Wall

Application
Flow Cytometry
Sample
Escherichia coli Cell (Planktonic)
Permeabilization
Yes - acetone
Specification
Planktonic
Fixation
Acetone
Username

Abcam user community

Verified customer

投稿 Aug 17 2015

Thank you for your enquiry.

It might be possible for a FITC-conjugated IgG to still be able to bind to Protein A as well, primarily due to the fact that FITC conjugation to IgG and Protein A binding to IgG occur at different sites within the...

Read More

Thank you for your patience. I can now confirm that ab30522 also cross-reacts with the DH5a strain of E.coli. I hope this information is helpful for your customer.

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Escherichia coli Cell (pure culture)
Specification
pure culture
Username

Abcam user community

Verified customer

投稿 May 09 2007

Thank you for your patience. Regarding ab30522 (E.coli antibody (FITC)), a mixed culture of E. coli strains was gamma-inactivated and used directly as the immunogen. Although the concentration has not been determined, there is a dilution recommend...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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