Anti-E Cadherin (phospho S838 + S840) 抗体 [EP913(2)Y] (ab76319)

製品の概要

  • 製品名Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y]
    E Cadherin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP913(2)Y] to E Cadherin (phospho S838 + S840)
  • 特異性Detects E Cadherin phosphorylated at serine 838 and 840.
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human E Cadherin.

  • ポジティブ・コントロール
    • WB: Human brain lysate; IHC-P: Human breast carcinoma or cervical carcinoma tissue.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500000. Predicted molecular weight: 97 kDa.
IP 1/50.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

  • 追加情報Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    • 機能Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
      E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
    • 組織特異性Non-neural epithelial tissues.
    • 関連疾患Defects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
      Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
      Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    • 配列類似性Contains 5 cadherin domains.
    • 翻訳後修飾During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
    • 細胞内局在Cell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Arc 1 antibody
      • CADH1_HUMAN antibody
      • Cadherin 1 antibody
      • cadherin 1 type 1 E-cadherin antibody
      • Cadherin1 antibody
      • CAM 120/80 antibody
      • CD 324 antibody
      • CD324 antibody
      • CD324 antigen antibody
      • cdh1 antibody
      • CDHE antibody
      • E-Cad/CTF3 antibody
      • E-cadherin antibody
      • ECAD antibody
      • Epithelial cadherin antibody
      • epithelial calcium dependant adhesion protein antibody
      • LCAM antibody
      • Liver cell adhesion molecule antibody
      • UVO antibody
      • Uvomorulin antibody
      see all

    Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] 画像

    • Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319) at 1/500000 dilution (purified) + Mouse brain at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 97 kDa
      Observed band size : 145 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319) at 1/500000 dilution (purified) + Rat brain at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 97 kDa
      Observed band size : 145 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319) at 1/500000 dilution (purified)

      Lane 1 : Untreated human fetal brain
      Lane 2 : Human fetal brain treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 97 kDa
      Observed band size : 145 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin embedded human skin with purified ab76319 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • ab76319 (purified) at 1/50 immunoprecipitating E cadherin in human fetal brain (Lane 1). For western blotting, a HRP-conjugated anti-rabbit IgG was used as the secondary antibody (1/1000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319) at 1/1000000 dilution (unpurified)

      Lane 1 : Human brain lysate, untreated
      Lane 2 : Human brain lysate treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 97 kDa
    • Unpurified ab76319 staining E Cadherin in mouse skin (pilosebaceous untis) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 10% normal donkey serum + 1% serum for 40 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6. Samples were incubated with primary antibody (1/400 in 1% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

      See Abreview

    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma using unpurified ab76319 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using unpurified ab76319 at 1/100 dilution.

    Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319) 使用論文

    This product has been referenced in:
    • Szeto HH  et al. Improving mitochondrial bioenergetics under ischemic conditions increases warm ischemia tolerance in the kidney. Am J Physiol Renal Physiol 308:F11-21 (2015). Read more (PubMed: 25339695) »
    • Nicholson TB  et al. A hypomorphic lsd1 allele results in heart development defects in mice. PLoS One 8:e60913 (2013). WB, IHC-P ; Mouse . Read more (PubMed: 23637775) »

    See all 4 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
    Sample Mouse Tissue sections (mouse skin, pilosebaceous units)
    Specification mouse skin, pilosebaceous units
    Permeabilization Yes - Tween-20
    Fixative Paraformaldehyde
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    投稿 Sep 15 2014

    I've been following up on the issues that we spoke about earlier in regards to the Anti-E Cadherin (phospho S838 + S840) antibody. I have also received your corresponence and thank you for taking the time to send that to me. I'll try to answer all the ...

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