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Mouse embryonal carcinoma cell line PCC4 Aza RI.
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Our Abpromise guarantee covers the use of ab11512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 - 10 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 20521328ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|WB||Use at an assay dependent concentration. PubMed: 19586906|
|IHC-Fr||Use at an assay dependent concentration.|
ICC/IF image of ab11512 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab11512 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Formaldehyde fixed (4%) (10 min) MCF-7 cells
ab11512 at 1/500 staining dog kidney cells by ICC/IF. The cells were methanol fixed and blocked with BSA before incubation with the antibody for 18 hours at 4°C. An Alexa Fluor ® 555 conjugated goat anti-rat IgG was used as the secondary.
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