製品の概要

  • 製品名Anti-E Cadherin antibody
    E Cadherin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to E Cadherin
  • アプリケーション適用あり: WB, ICC, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human, Pig
  • 免疫原

    Recombinant fragment:

    IFFCERNPKP QVINIIDADL PPNTSPFTAE LTHGASANWT IQYNDPTQES IILKPKMALE VGDYKINLKL MDNQNKDQVT TLEVSVCDCE GAAGVCRKAQ PVEAGLQI

    , corresponding to amino acids 600-707 of Human E Cadherin.

  • ポジティブ・コントロール
    • Skin

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 120 kDa.
ICC Use at an assay dependent concentration.
IHC-P 1/30. for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
IHC-Fr 1/50.

ターゲット情報

  • 機能Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
    E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
  • 組織特異性Non-neural epithelial tissues.
  • 関連疾患Defects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
    Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
    Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
  • 配列類似性Contains 5 cadherin domains.
  • 翻訳後修飾During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
  • 細胞内局在Cell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Arc 1 antibody
    • CADH1_HUMAN antibody
    • Cadherin 1 antibody
    • cadherin 1 type 1 E-cadherin antibody
    • Cadherin1 antibody
    • CAM 120/80 antibody
    • CD 324 antibody
    • CD324 antibody
    • CD324 antigen antibody
    • cdh1 antibody
    • CDHE antibody
    • E-Cad/CTF3 antibody
    • E-cadherin antibody
    • ECAD antibody
    • Epithelial cadherin antibody
    • epithelial calcium dependant adhesion protein antibody
    • LCAM antibody
    • Liver cell adhesion molecule antibody
    • UVO antibody
    • Uvomorulin antibody
    see all

Anti-E Cadherin antibody 画像

  • All lanes : Anti-E Cadherin antibody (ab15148) at 1/500 dilution (for 16 hours at 4°C)

    Lane 1 : Human OE33 cell - whole cell lysate
    Lane 2 : Human OE33 cell - whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 120 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% BSA fro 1 hour at 23°C

    See Abreview

  • ab15148 staining E Cadherin in Human breast cancer MDA-MB-231 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were treated with ehanol (vehicle) as control or Origanummarjorana extract for 24 hours. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature. Samples were incubated with primary antibody overnight at 4°C. An Alexa Fluor 488-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/200) was used as the secondary antibody.

  • ab15148 staining E Cadherin in Human AGS Gastric Carcinoma tissue sections by Immunohistochemistry (Frozen sections). The sections were acetone fixed and blocked in 5% serum for 1 hour at 23°C. The primary antibody was diluted 1/50 in blocking buffer and incubated with the sample for 1 hours at 23°C.  An HRP-conjugated Goat polyclonal to Rabbit IgG, diluted 1/200, was used as the secondary.

    See Abreview

  • ab15148 staining human MCF10A cells by ICC/IF.  Cells were fixed with paraformaldehyde and blocked using 10% serum for 30 minutes at 25 °C.  The primary antibody was diluted 1/25 in TBST and incubated for 1 hour at 25 °C.  An Alexa Fluor® 555 goat anti-rabbit was used as the secondary antibody.

    See Abreview

  • ab15148 staining E Cadherin in Pig Cervix uteri tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% goat serum for 1 hour at 37°C; antigen retrieval was by heat mediation in 10mM citrate at pH 6 for 2 minutes. The sample was incubated with primary antibody (1/50) at 4°C for 12 hours. An HRP-conjugated goat polyclonal to rabbit IgG (undiluted) was used as secondary antibody.

    See Abreview

  • Immunohistochemical staining of formalin fixed paraffin embedded human skin using ab15148.
  • ab15148 staining E Cadherin in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was used diluted 1/50 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.

    See Abreview

  • ab15148 staining E cadherin in Human AGS Gastric carcinoma cultured cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilzed with 0.025% Trton X-100 in TBS and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/50 in blocking buffer) for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

Anti-E Cadherin antibody (ab15148) 使用論文

This product has been referenced in:
  • Nguyen DG  et al. Bioprinted 3D Primary Liver Tissues Allow Assessment of Organ-Level Response to Clinical Drug Induced Toxicity In Vitro. PLoS One 11:e0158674 (2016). Read more (PubMed: 27387377) »
  • You A  et al. Metformin sensitizes sorafenib to inhibit postoperative recurrence and metastasis of hepatocellular carcinoma in orthotopic mouse models. J Hematol Oncol 9:20 (2016). WB ; Human . Read more (PubMed: 26957312) »

See all 24 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (Swine testicle cells)
Permeabilization Yes - Triton, 0.1%, 2min
Specification Swine testicle cells
Fixative Formaldehyde
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投稿 Dec 15 2015

Application ELISA
Sample Human Cell (H9 embryonic stem cells)
Specification H9 embryonic stem cells
Type Indirect
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Oct 11 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Human Cell (H9 embryonic stem cells)
Specification H9 embryonic stem cells
Permeabilization Yes - 0.01% Triton X100
Fixative Formaldehyde
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投稿 Oct 04 2013

Application Western blot
Sample Human Cell lysate - whole cell (MCF-7)
Loading amount 100000 cells
Specification MCF-7
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Nov 19 2012

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
The details you have kindly provided will enable us to investigate this case ...

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Thank you for contacting us.
The immunogen of ab53033 corresponds to the internal sequence of E-cadherin so this may not suitable as per your requirements.
The immunogens of antibodies ab15148, ab77287, ab128804, and ab133597 are located withi...

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Thank you for your phone call. Our current E Cadherin antibodies that bind to an extracellular domain of the human protein are:

ab8050
ab14015
ab15148
ab15150
ab22585
ab40772
ab45990
ab77287

I hope...

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Thank you for confirming this information and for your help and cooperation with this case. As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order. As ...

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Thank you for confirming this information and for your help and cooperation with this case. As mentioned before, the protocol looks absolutely fine to me and the results with ab53033 are excellent. I can suggest the customer has regrettably receive...

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Thank you for contacting us. I am sorry to hear you have been experiencing problems with one of our antibodies. The quality of our products is important to us and I would like to reassure you that we investigate all customer complaints. I appreci...

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1-10 of 22 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"