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Our Abpromise guarantee covers the use of ab15278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunohistochemical staining of human skeletal muscle with ab15278
ab15278 staining Dystrophin in transverse sections of tibialis anterior (TA) muscles of mdx mice by Immunohistochemistry (Frozen sections). Mice were killed and TA muscles were removed, pinned to parafilm-covered cork, snap frozen in liquid N2-cooled isopentane, and stored at -80°C. Sections were cut using cryostat and blocked with 10% normal goat serum in 1% BSA/PBS for 1 h and then incubated with primary antibody for 1 hour. A Cy3 conjugated anti rabbit IgG at 1/500 dilution was used as secondary. Slides were coverslipped with Vectashield mounting medium with DAPI and imaged.
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