製品の概要

  • 製品名Anti-DYRK2 antibody
    DYRK2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DYRK2
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    KLH-conjugated synthetic peptide selected from the sequence of Human DYRK2 within N terminal amino acids 1-100.

  • ポジティブ・コントロール
    • 293 cell lysate, Human breast carcinoma.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)This antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab37912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能Role in the regulation of cellular growth and/or development. Regulates TP53 by phosphorylation on Ser-46 to induce apoptosis in response to DNA damage, functioning downstream of ATM. Inactivates GYS1 by phosphorylation at Ser-641, and potentially also a second phosphorylation site, thus regulating glycogen synthesis. Phosphorylates EIF2B5 at Ser-544, enabling its subsequent phosphorylation and inhibition by GSK3, and may play a more general role in the priming of GSK3 substrates.
  • 組織特異性Testis, after the onset of spermatogenesis.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylated on serine/threonine residues.
  • 細胞内局在Cytoplasm. Nucleus. Translocates into the nucleus following DNA damage.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1810038L18Rik antibody
    • Dual specificity tyrosine (Y) phosphorylation regulated kinase 2 antibody
    • Dual specificity tyrosine phosphorylation regulated kinase 2 antibody
    • Dual specificity tyrosine-phosphorylation-regulated kinase 2 antibody
    • DYRK2 antibody
    • DYRK2_HUMAN antibody
    • EC 2.7.12.1 antibody
    • FLJ21217 antibody
    • FLJ21365 antibody
    see all

Anti-DYRK2 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling DYRK2 with ab37912 at 1/25 dilution. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Anti-DYRK2 antibody (ab37912) at 1/1000 dilution + HEK 293 whole cell lysate at 35 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 17 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-DYRK2 antibody (ab37912) 使用論文

This product has been referenced in:
  • Maddika S & Chen J Protein kinase DYRK2 is a scaffold that facilitates assembly of an E3 ligase. Nat Cell Biol 11:409-19 (2009). WB ; Human . Read more (PubMed: 19287380) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Pancreatic)
Loading amount 37.5 µg
Specification Pancreatic
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Nov 05 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"